Human AMPK alpha 2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human AMPK alpha 2 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human AMPK alpha 1 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 847123
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human AMPK alpha 2
    Phe340-Arg552
    Accession # P54646
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Simple Western
    20 µg/mL
    See below
  • Immunocytochemistry
    5-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human AMPK alpha 2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑468 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human AMPK alpha 2 Monoclonal Antibody (Catalog # MAB2850) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for AMPK alpha 2 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human AMPK alpha 2 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for AMPK alpha 2 at approximately 71 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human AMPK alpha 2 Monoclonal Antibody (Catalog # MAB2850). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Immunocytochemistry
AMPK alpha 2 in HEK293 Human Cell Line. AMPK alpha 2 was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human AMPK alpha 2 Monoclonal Antibody (Catalog # MAB2850) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AMPK alpha 2
AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and δ subunits. The alpha subunit of AMPK is activated allosterically by AMP, and by phosphorylation via the AMPK kinase LKB1. Active AMPK down-regulates anabolic pathways such as fatty acid and cholesterol synthesis, and up-regulates catabolic pathways such as glycolysis and fatty acid oxidation.
  • Long Name:
    AMP-activated Protein Kinase alpha
  • Entrez Gene IDs:
    5563 (Human); 108079 (Mouse); 78975 (Rat)
  • Alternate Names:
    5'-AMP-activated protein kinase catalytic subunit alpha-2; AMPK alpha 2; AMPK subunit alpha-2; AMPK2; AMPK5'-AMP-activated protein kinase, catalytic alpha-2 chain; AMPK-alpha-2 chain; EC 2.7.11; EC 2.7.11.1; PRKAA; PRKAA2; protein kinase, AMP-activated, alpha 2 catalytic subunit
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