Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat, Avian - Chicken

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 94408
View all APAF-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human APAF-1
Leu10-Asp214
Accession # AAC51678

Specificity

Detects human APAF-1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human APAF‑1 Antibody

Detection of Human APAF‑1 antibody by Western Blot.

Detection of Human APAF‑1 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. Gels were loaded with 2 x 105cells (lane 1) and 7 x 104cells (lane 2). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human APAF-1 Monoclonal Antibody (Catalog # MAB868) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for APAF-1 was detected at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Applications for Human APAF‑1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: APAF-1

Apoptosis protease-activating factor 1 (APAF‑1) is a ubiquitous 135 kDa protein component of the cytoplasmic apoptosome complex. APAF‑1 plays an important role in the mitochondrial apoptotic pathway by triggering the Cytochrome c-dependent activation of Caspase-9. APAF‑1 participates in tissue morphogenesis during development, and loss of APAF‑1 expression is associated with tumor progression in a variety of cancers. Some alternate splice forms of APAF‑1 are deficient in their ability to promote apoptosis.

Long Name

Apoptotic Peptidase Activating Factor 1

Alternate Names

APAF1

Entrez Gene IDs

317 (Human)

Gene Symbol

APAF1

UniProt

AAC51678

Additional APAF-1 Products

Product Documents for Human APAF‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human APAF‑1 Antibody

For research use only

Related Research Areas

Cysteine Proteases and Regulators

Citations for Human APAF‑1 Antibody

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail