Human ASC Antibody

Detection of ASC by Western Blot

LPS induced cell pyroptosis, apoptosis and pro-inflammatory cytokines secretion in both IUA mice and cellular models. The expression status of (a) NLRP3, ASC and N-Gasdermin D, and (b) cleaved caspase-3 and Bax were detected by performing Western blot analysis. The pro-inflammatory cytokines generation and secretion were examined by (c) Real-Time qPCR in EPCs cells and (d-g) its supernatants were examined by Real-Time qPCR and ELISA assay. (h) Western blot was used to determine cell pytoptosis in EPCs cells. (i) MTT assay was used to determine cell viability in EPCs cells. (j) Generation and (k, l) secretion of IL-1 beta and IL-18 were respectively examined by Real-Time qPCR and ELISA. Individual experiment repeated 3 times, and *P < 0.05 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34738867), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of ASC by Western Blot

MiR-223-3p was capable of regulating NLRP3-mediated pyroptotic cell death in EPCs. (a) The eight miRNAs in BMSCs-exo were screened by using Real-Time qPCR, and (b) the process that BMSCs-exo delivered miR-223-3p into EPCs was examined. (c) The targeting sites in miR-223-3p and 3ʹ UTR of NLRP3 mRNA were predicted, and (d) those sites were validated by performing dual-luciferase reporter gene system assay. (e) miR-223-3p was overexpressed and silenced, and (f, g) the following experiments validated that miR-223-3p negatively regulated NLRP3 in EPCs cells. Upregulation of miR-223-3p suppressed LPS-induced (h) pyroptotic cell death, (i) cytokines generation and (j, k) secretion in EPCs cells. Individual experiment repeated 3 times, and *P < 0.05 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34738867), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of ASC by Western Blot

LPS induced cell pyroptosis, apoptosis and pro-inflammatory cytokines secretion in both IUA mice and cellular models. The expression status of (a) NLRP3, ASC and N-Gasdermin D, and (b) cleaved caspase-3 and Bax were detected by performing Western blot analysis. The pro-inflammatory cytokines generation and secretion were examined by (c) Real-Time qPCR in EPCs cells and (d-g) its supernatants were examined by Real-Time qPCR and ELISA assay. (h) Western blot was used to determine cell pytoptosis in EPCs cells. (i) MTT assay was used to determine cell viability in EPCs cells. (j) Generation and (k, l) secretion of IL-1 beta and IL-18 were respectively examined by Real-Time qPCR and ELISA. Individual experiment repeated 3 times, and *P < 0.05 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34738867), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of ASC by Western Blot

(A) NLRP3 inflammasome protein levels are displayed by Western blot in three different fibroblast cell populations: healthy fibroblasts (Healthy), psoriatic fibroblasts (PSO), and psoriatic fibroblasts infected with a pRRL-TTP-overexpressing vector (PSO TTP). Tubulin was used as loading control. (B) TTP overexpression is shown in psoriatic fibroblast sample. Actin was used as loading control. (C) Inflammasome components variation pattern already seen in primary fibroblasts is maintained in the HaCaT keratinocyte cell line. Actin was used as loading control. (D) pRRL-empty vector was used to obtain the overexpression vector pRRL-TTP, by substitution of delta NGFR sequence with TTP cDNA. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33585499), licensed under a CC-BY license. Not internally tested by R&D Systems.