Human ATF4 Antibody

Catalog # Availability Size / Price Qty
Detection of Human ATF4 by Western Blot.
2 Images
Product Details
Citations (1)
Supplemental Products
Reviews (2)

Human ATF4 Antibody Summary

Species Reactivity
Detects human ATF4 in direct ELISAs.
Monoclonal Mouse IgG2A Clone # 739441
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human ATF4
Accession # P18848
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Complete Your Experiment


Recommended Concentration
Western Blot
2 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ATF4 antibody by Western Blot. View Larger

Detection of Human ATF4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ATF4 Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB7218) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ATF4 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry ATF4 antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC). View Larger

ATF4 in Jurkat Human Cell Line. ATF4 was detected in immersion fixed Jurkat human acute T-cell leukemia cell line using Mouse Anti-Human ATF4 Monoclonal Antibody (Catalog # MAB7218) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATF4

ATF4 is an approximately 38 kDa member of the bzip family of transcription factors. It plays an important role autophagy, the unfolded protein response, and the response to oxygen and nutrient deprivation. These functions are regulated by the dimerization of ATF4 with the leucine zipper protein FIAT. ATF4 contains a basic DNA-binding domain (aa 280-300) and a leucine zipper domain (aa 306-334). Human ATF4 shares 87% aa sequence identity with mouse and rat ATF4.

Long Name
Activating Transcription Factor 4
Entrez Gene IDs
468 (Human); 11911 (Mouse); 79255 (Rat)
Alternate Names
activating transcription factor 4 (tax-responsive enhancer element B67); Activating transcription factor 4; ATF4; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB-2DNA-binding protein TAXREB67; cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; TAXREB67; TAXREB67CREB2cAMP response element-binding protein 2; Tax-responsive enhancer element-binding protein 67; TXREB

Product Datasheets

You must select a language.


Citation for Human ATF4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Epigenetic Priming with Decitabine Augments the Therapeutic Effect of Cisplatin on Triple-Negative Breast Cancer Cells through Induction of Proapoptotic Factor NOXA
    Authors: W Nakajima, K Miyazaki, M Sakaguchi, Y Asano, M Ishibashi, T Kurita, H Yamaguchi, H Takei, N Tanaka
    Cancers, 2022;14(1):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human ATF4 Antibody

Average Rating: 3 (Based on 2 Reviews)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Human ATF4 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Human ATF4 Antibody
By Anonymous on 12/28/2021
Application: ICC/IF Sample Tested: Jurkat human acute T-cell leukemia cell line Species: Human

Human ATF4 Antibody
By Anonymous on 11/29/2017
Application: Simple Western Sample Tested: HCT-116 human colorectal carcinoma cell line Species: Human

Technical Service will be following up
This antibody was tested in an unvalidated application