Human ATF4 Antibody

(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human ATF4 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2A Clone # 739441
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human ATF4
    Met1-Pro351
    Accession # P18848
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human ATF4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ATF4 Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB7218) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ATF4 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
ATF4 in Jurkat Human Cell Line. ATF4 was detected in immersion fixed Jurkat human acute T-cell leukemia cell line using Mouse Anti-Human ATF4 Monoclonal Antibody (Catalog # MAB7218) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ATF4
ATF4 is an approximately 38 kDa member of the bzip family of transcription factors. It plays an important role autophagy, the unfolded protein response, and the response to oxygen and nutrient deprivation. These functions are regulated by the dimerization of ATF4 with the leucine zipper protein FIAT. ATF4 contains a basic DNA-binding domain (aa 280-300) and a leucine zipper domain (aa 306-334). Human ATF4 shares 87% aa sequence identity with mouse and rat ATF4.
  • Long Name:
    Activating Transcription Factor 4
  • Entrez Gene IDs:
    468 (Human); 11911 (Mouse); 79255 (Rat)
  • Alternate Names:
    activating transcription factor 4 (tax-responsive enhancer element B67); Activating transcription factor 4; ATF4; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB-2DNA-binding protein TAXREB67; cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; TAXREB67; TAXREB67CREB2cAMP response element-binding protein 2; Tax-responsive enhancer element-binding protein 67; TXREB
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Average Rating: 1 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Simple Western.

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Images Ratings Applications Species Reviewed By Date Details
Simple Western Human ATF4 Antibody MAB7218
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 Simple Western Human Anonymous 11/29/2017
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Simple Western Human ATF4 Antibody MAB7218
Simple Western: Human ATF4 Antibody [MAB7218]

Summary

ApplicationSimple Western
Sample TestedHCT-116 human colorectal carcinoma cell line
SpeciesHuman

Other Experimental Details

Other Experimental DetailsTechnical Service will be following upThis antibody was tested in an unvalidated application

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