Human ATF4 Antibody

Catalog # Availability Size / Price Qty
MAB7218
MAB7218-SP
Detection of Human ATF4 by Western Blot.
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Product Details
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Human ATF4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ATF4 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 739441
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ATF4
Met1-Pro351
Accession # P18848
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human ATF4 antibody by Western Blot. View Larger

Detection of Human ATF4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ATF4 Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB7218) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ATF4 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry ATF4 antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC). View Larger

ATF4 in Jurkat Human Cell Line. ATF4 was detected in immersion fixed Jurkat human acute T-cell leukemia cell line using Mouse Anti-Human ATF4 Monoclonal Antibody (Catalog # MAB7218) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATF4

ATF4 is an approximately 38 kDa member of the bzip family of transcription factors. It plays an important role autophagy, the unfolded protein response, and the response to oxygen and nutrient deprivation. These functions are regulated by the dimerization of ATF4 with the leucine zipper protein FIAT. ATF4 contains a basic DNA-binding domain (aa 280-300) and a leucine zipper domain (aa 306-334). Human ATF4 shares 87% aa sequence identity with mouse and rat ATF4.

Long Name
Activating Transcription Factor 4
Entrez Gene IDs
468 (Human); 11911 (Mouse); 79255 (Rat)
Alternate Names
activating transcription factor 4 (tax-responsive enhancer element B67); Activating transcription factor 4; ATF4; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB-2DNA-binding protein TAXREB67; cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; TAXREB67; TAXREB67CREB2cAMP response element-binding protein 2; Tax-responsive enhancer element-binding protein 67; TXREB

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human ATF4 Antibody

Average Rating: 3 (Based on 2 Reviews)

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Human ATF4 Antibody
By Anonymous on 12/28/2021
Application: ICC/IF Sample Tested: Jurkat human acute T-cell leukemia cell line Species: Human

Human ATF4 Antibody
By Anonymous on 11/29/2017
Application: Simple Western Sample Tested: HCT-116 human colorectal carcinoma cell line Species: Human

Technical Service will be following up
This antibody was tested in an unvalidated application