Human B7-H4 Antibody MAB6576: R&D Systems

Human B7-H4 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Stains human B7-H4 transfectants but not irrelevant transfectants in flow cytometry.
  • Source
    Monoclonal Mouse IgG2B Clone # 935317
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human B7-H4
    Phe29-Ser258
    Accession # Q727D3
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of B7‑H4 in HEK293 Human Cell Line Transfected with Human B7-H4 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human B7-H4 or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human B7‑H4 Monoclonal Antibody (Catalog # MAB6576) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: B7-H4
B7-H4, also known as VTCN1, B7x and B7S1, is a 50‑80 kDa glycosylated member of the BTN/MOG family of immunomodulatory protein (1, 2). Mature human B7-H4 consists of a 235 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and one Ig-like C2-set domain, a 21 aa transmembrane segment, and a 2 aa cytoplasmic tail (3-5). Within the ECD, human B7-H4 shares 90% aa sequence identity with mouse and rat B7-H4. It shares 22%-28% aa sequence identity with human B7-1, B7-2, B7-H1, B7-H2, B7-H3, and PD‑L2. Alternate splicing of human B7-H4 generates an additional isoform that lacks the first Ig-like domain. B7-H4 is expressed on the surface of activated lymphocytes, macrophages, monocytes, dendritic cells, epithelial cells, and bone marrow-derived mesenchymal stem cells
(4-8). Following binding to activated T cells, B7-H4 serves as a co‑inhibitor of the T cell response. This is accomplished by reverse signaling that can induce either cell cycle arrest, or apoptosis in B7-H4 expressing cells (3-5, 9, 10). B7‑H4 is up‑regulated in several carcinomas in correlation with tumor progression and metastasis (2, 7, 11, 12). A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status (13-15). Soluble B7‑H4 functions as a decoy molecule that blocks the inhibitory influence of B7‑H4 on immune activation (15). Despite evidence for the involvement of B7-H4 in immune regulation, mice deficient in its expression do not show significant immune deficiencies, suggesting compensation by other molecules in vivo (16).
  • References:
    1. Yi, K.H. and L. Chen (2009) Immunol. Rev. 229:145.
    2. Salceda, S. et al. (2005) Exp. Cell Res. 306:128.
    3. Zang, X. et al. (2003) Proc. Natl. Acad. Sci. 100:10388.
    4. Prasad, V.R. et al. (2003) Immunity 18:863.
    5. Sica, G.L. et al. (2003) Immunity 18:849.
    6. Kryczek, I. et al. (2006) J. Exp. Med. 203:871.
    7. Tringler, B. et al. (2005) Clin. Cancer Res. 11:1842.
    8. Xue, Q. et al. (2010) Stem Cells Dev. 19:27.
    9. Song, H. et al. (2008) Cancer Lett. 266:227.
    10. Park, G.B. et al. (2009) Immunology 128:360.
    11. Zang, X. et al. (2007) Proc. Natl. Acad. Sci. 104:19458.
    12. Krambeck, A.E. et al. (2006) Proc. Natl. Acad. Sci. 103:10391.
    13. Simon, I. et al. (2006) Cancer Res. 66:1570.
    14. Thompson, R.H. et al. (2008) Cancer Res. 68:6054.
    15. Azuma, T. et al. (2009) PloS Med. 6:e1000166.
    16. Suh, W.-K. et al. (2006) Mol. Cell. Biol. 26:6403.
  • Long Name:
    B7 Homolog 4
  • Entrez Gene IDs:
    79679 (Human); 242122 (Mouse)
  • Alternate Names:
    B7h.5; B7H4; B7-H4; B7H4T-cell costimulatory molecule B7x; B7S1; B7S1VCTN1; B7x; B7XPRO1291; FLJ22418; Immune costimulatory protein B7-H4; Protein B7S1; T cell costimulatory molecule B7x; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; Vtcn1
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