Human beta -3 Adrenergic R/ADRB3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Stains human beta -3 Adrenergic R/ADRB3 transfectants but not irrelevent transfectants in flow cytometry.
  • Source
    Monoclonal Mouse IgG2B Clone # 493324
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human embryonic kidney cell line HEK293-derived transfected with human beta -3 Adrenergic R/ADRB3
    Met1-Ser408
    Accession # P13945
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of beta ‑3 Adrenergic R/ADRB3 in HEK293 Human Cell Line Transfected with Human beta ‑3 Adrenergic R/ADRB3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human beta ‑3 Adrenergic R/ADRB3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human beta ‑3 Adrenergic R/ADRB3 Monoclonal Antibody (Catalog # MAB9419) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry
beta ‑3 Adrenergic R/ADRB3 in Human Adipocytes. beta ‑3 Adrenergic R/ADRB3 was detected in immersion fixed human adipocytes using Mouse Anti-Human beta ‑3 Adrenergic R/ADRB3 Monoclonal Antibody (Catalog # MAB9419) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-3 Adrenergic R/ADRB3
 ADRB3 belongs to the G protein-coupled receptor 1 family of beta adrenergic receptors. Beta-adrenergic receptors, including ADRB3, mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. ADRB3 is involved in the regulation of lipolysis and thermogenesis. A variant of ADRB3 seems to be associated with weight gain (obesity).
  • Long Name:
    Beta-3 Adrenergic Receptor
  • Entrez Gene IDs:
    155 (Human); 11556 (Mouse); 25645 (Rat)
  • Alternate Names:
    ADRB3; ADRB3R; adrenergic, beta-3-, receptor; B3AR; beta-3 Adrenergic R; beta-3 adrenergic receptor; beta3 AdrenergicR; beta-3 AdrenergicR; Beta-3 adrenoceptor; Beta-3 adrenoreceptor; BETA3AR; FLJ99960
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