Detection of beta ‑3 Adrenergic R/ADRB3 in HEK293 Human Cell Line Transfected with Human beta ‑3 Adrenergic R/ADRB3 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human beta ‑3 Adrenergic R/ADRB3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human beta ‑3 Adrenergic R/ADRB3 Monoclonal Antibody (Catalog # MAB9419) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.
beta ‑3 Adrenergic R/ADRB3 in Human Adipocytes.
beta ‑3 Adrenergic R/ADRB3 was detected in immersion fixed human adipocytes using Mouse Anti-Human beta ‑3 Adrenergic R/ADRB3 Monoclonal Antibody (Catalog # MAB9419) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-3 Adrenergic R/ADRB3
ADRB3 belongs to the G protein-coupled receptor 1 family of beta adrenergic receptors. Beta-adrenergic receptors, including ADRB3, mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. ADRB3 is involved in the regulation of lipolysis and thermogenesis. A variant of ADRB3 seems to be associated with weight gain (obesity).
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