Key Product Details

Species Reactivity

Human

Applications

Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all BMI-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human BMI-1
Asp96-Gly326
Accession # P35226

Specificity

Detects human BMI-1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human BMI‑1 Antibody

BMI‑1 antibody in K562 Human Cell Line by Immunocytochemistry (ICC).

BMI‑1 in K562 Human Cell Line.

BMI-1 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using 10 µg/mL Goat Anti-Human BMI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3334) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human BMI‑1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

0.1 µg/mL
Sample: Recombinant Human BMI‑1

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: BMI-1

BMI-1 (B cell-specific Moloney-MLV Integration site 1) is a 45 kDa protooncogene that is a class II member of the Polycomb group of genes. It participates in the formation of large multimeric complex termed PRC1 that inhibits target gene transcription loss of BMI-1 function and precludes stem cells from self-replicating. The amino acid sequence of human BMI-1 is 99%, 97%, 99%, and 99% identical to that of bovine, mouse, feline, and canine BMI-1, respectively.

Long Name

B Lymphoma Mo-MLV Insertion Region 1

Alternate Names

BMI1, PCGF4, RNF51

Entrez Gene IDs

648 (Human); 12151 (Mouse)

Gene Symbol

BMI1

UniProt

P35226

Additional BMI-1 Products

Product Documents for Human BMI‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human BMI‑1 Antibody

For research use only

Customer Reviews for Human BMI‑1 Antibody

There are currently no reviews for this product. Be the first to review Human BMI‑1 Antibody and earn rewards!

Have you used Human BMI‑1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies