Detection of Human BNIP3 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 150 µM CoCl2 for 18 hours. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human BNIP3 Monoclonal Antibody (Catalog # MAB4147) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BNIP3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
E1B 19 kDa protein-interacting protein 3 (BNIP3), also known as 19 kDa
interacting protein 3 (NIP3), is a proapoptotic member of Bcl-2 protein
family. BNIP3 is a 194 amino acid, 21.5 kDa (predicted) protein that
contains a single Bcl-2 homology 3 (BH3) domain and a C-terminal
transmembrane domain required for mitochondrial localization,
homodimerization, and regulation of its proapoptotic function. BNIP3 was
identified as one of several proteins that interact with discrete
domains of Bcl-2 and the E1B 19 kDa protein. Under conditions of
prolonged oxygen deprivation, the hypoxia-induced protein HIF1-alpha
activates expression of BNIP3, which in turn, promotes apoptosis under
these conditions. The mechanism of BNIP3-mediated apoptosis is
independent of caspase activation and cytochrome c release and is
characterized by early plasma membrane and mitochondrial damage, prior
to the appearance of chromatin condensation or DNA fragmentation. Human
BNIP3 shares 90% amino acid identity with mouse and rat BNIP3. Human
BNIP3 shares 56% amino acid sequence identity with human BNIP3L.
BCL2/Adenovirus E1B 19 Kda Protein-Interacting Protein 3-Like
Entrez Gene IDs:
664 (Human); 12176 (Mouse); 84480 (Rat)
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BCL2/adenovirus E1B 19kDa interacting protein 3; BCL2/adenovirus E1B 19kD-interacting protein 3; Nip3
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