Human BNIP3 Antibody

Catalog # Availability Size / Price Qty
Detection of Human BNIP3 by Western Blot.
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Product Details
Citations (2)
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Human BNIP3 Antibody Summary

Species Reactivity
Detects human BNIP3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human BNIP3L is observed.
Monoclonal Mouse IgG2B Clone # 670621
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human BNIP3
Accession # Q12983
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human BNIP3 antibody by Western Blot. View Larger

Detection of Human BNIP3 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 150 µM CoCl2for 18 hours. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human BNIP3 Monoclonal Antibody (Catalog # MAB4147) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BNIP3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BNIP3

Bcl-2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3), also known as 19 kDa interacting protein 3 (NIP3), is a proapoptotic member of Bcl-2 protein family. BNIP3 is a 194 amino acid, 21.5 kDa (predicted) protein that contains a single Bcl-2 homology 3 (BH3) domain and a C-terminal transmembrane domain required for mitochondrial localization, homodimerization, and regulation of its proapoptotic function. BNIP3 was identified as one of several proteins that interact with discrete domains of Bcl-2 and the E1B 19 kDa protein. Under conditions of prolonged oxygen deprivation, the hypoxia-induced protein HIF1-alpha activates expression of BNIP3, which in turn, promotes apoptosis under these conditions. The mechanism of BNIP3-mediated apoptosis is independent of caspase activation and cytochrome c release and is characterized by early plasma membrane and mitochondrial damage, prior to the appearance of chromatin condensation or DNA fragmentation. Human BNIP3 shares 90% amino acid identity with mouse and rat BNIP3. Human BNIP3 shares 56% amino acid sequence identity with human BNIP3L.

Long Name
BCL2/Adenovirus E1B 19 Kda Protein-Interacting Protein 3-Like
Entrez Gene IDs
664 (Human); 12176 (Mouse); 84480 (Rat)
Alternate Names
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BCL2/adenovirus E1B 19kDa interacting protein 3; BCL2/adenovirus E1B 19kD-interacting protein 3; BNIP3; Nip3

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Citations for Human BNIP3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. High content screening identifies monensin as an EMT-selective cytotoxic compound
    Authors: M Vanneste, Q Huang, M Li, D Moose, L Zhao, MA Stamnes, M Schultz, M Wu, MD Henry
    Sci Rep, 2019;9(1):1200.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Autophagy Induction Results in Enhanced Anoikis-resistance in Models of Peritoneal Disease
    Authors: John Hays
    Mol. Cancer Res., 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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