Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA Development (Detection)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Calbindin D Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Calbindin D
Ala2-Asn261
Accession # P05937

Specificity

Detects human Calbindin D in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Calbindin D Antibody

Detection of Human Calbindin D antibody by Western Blot.

Detection of Human Calbindin D by Western Blot.

Western blot shows human urine and lysates of human kidney tissue. PVDF membrane was probed with 5 µg/mL of Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Calbindin D at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Calbindin D antibody by Simple WesternTM.

Detection of Human Calbindin D by Simple WesternTM.

Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Calbindin D at approximately 32 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Calbindin D in Human Brain (Hippocampus).

Calbindin D was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and synaptic vesicles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Calbindin D Antibody

Application
Recommended Usage

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)

Simple Western

10 µg/mL
Sample: Human brain (cerebellum) tissue

Western Blot

5 µg/mL
Sample: Human urine and human kidney tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Calbindin D

Calbindin D, also known as Calbindin 1, is a Vitamin D-dependent cytoplasmic protein in the EF-hand calcium binding protein family that includes calmodulin, parvalbumin, troponin C, and S100. Calbindin D is widely expressed and regulates calcium homeostasis. Calbindin D protects neurons from excitotoxic and apoptotic cell death by buffering excess calcium. Human, mouse, and rat Calbindin D share 98% amino acid sequence identity.

Long Name

Vitamin D-dependent Calcium-Binding Protein

Alternate Names

CABP, CABP1, CABP28K, CABP9K, Calbindin 3, Calbindin-D, Calbindin-D9K, S100G

Entrez Gene IDs

795 (Human); 12309 (Mouse); 24249 (Rat)

Gene Symbol

S100G

UniProt

P05937

Additional Calbindin D Products

Product Documents for Human Calbindin D Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Calbindin D Antibody

For research use only

Citations for Human Calbindin D Antibody

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Protocols

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