Human Calbindin D Antibody

Catalog # Availability Size / Price Qty
AF3320
AF3320-SP
Human Calbindin D Antibody in Data
2 Images
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews

Human Calbindin D Antibody Summary

Species Reactivity
Human
Specificity
Detects human Calbindin D in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Calbindin D
Ala2-Asn261
Accession # P05937
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human brain (cerebellum), intestine, and rat brain (cerebellum)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Calbindin D by Western Blot. View Larger

Detection of Human Calbindin D by Western Blot. Western blot shows human urine and lysates of human kidney tissue. PVDF membrane was probed with 5 µg/mL of Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Calbindin D at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Calbindin D by Simple Western<sup>TM</sup>. View Larger

Detection of Human Calbindin D by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Calbindin D at approximately 32 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calbindin D

Calbindin D, also known as Calbindin 1, is a Vitamin D-dependent cytoplasmic protein in the EF-hand calcium binding protein family that includes calmodulin, parvalbumin, troponin C, and S100. Calbindin D is widely expressed and regulates calcium homeostasis. Calbindin D protects neurons from excitotoxic and apoptotic cell death by buffering excess calcium. Human, mouse, and rat Calbindin D share 98% amino acid sequence identity.

Long Name
Vitamin D-dependent Calcium-Binding Protein
Entrez Gene IDs
795 (Human); 12309 (Mouse); 24249 (Rat)
Alternate Names
CABP; CABP1; CABP28K; CABP9K; Calbindin 3; Calbindin D; Calbindin-D; Calbindin-D9K; S100G

Product Datasheets

Citations for Human Calbindin D Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Mechanically Activated Piezo Channels Mediate Touch and Suppress Acute Mechanical Pain Response in Mice
    Authors: M Zhang, Y Wang, J Geng, S Zhou, B Xiao
    Cell Rep, 2019;26(6):1419-1431.e4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Cerebrospinal fluid calbindin D concentration as a biomarker of cerebellar disease progression in Niemann-Pick type C1 disease
    Authors: Allison M Bradbury
    J Pharmacol Exp Ther, 2016;0(0):.
    Species: Human
    Sample Types: CSF
    Applications: ELISA Development (Detection)
  3. Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
    Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
    Nat Neurosci, 2015;18(1):145-53.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Rgs6 is required for adult maintenance of dopaminergic neurons in the ventral substantia nigra.
    Authors: Bifsha P, Yang J, Fisher R, Drouin J
    PLoS Genet, 2014;10(12):e1004863.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity.
    Authors: Di Giovannantonio, Luca G, Di Salvio, Michela, Omodei, Daniela, Prakash, Nilima, Wurst, Wolfgang, Pierani, Alessand, Acampora, Dario, Simeone, Antonio
    Development, 2014;141(2):377-88.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP.
    Authors: Di Salvio M, Di Giovannantonio LG, Acampora D
    Nat. Neurosci., 2010;13(12):1481-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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