Key Product Details

Species Reactivity

Validated:

Human, Canine

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 74902
View all NGFR/TNFRSF16 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NGF R
Lys29-Asn250
Accession # P08138

Specificity

Detects human NGF R in direct ELISAs and Western blots. In direct ELISAs, no crossreactivity with recombinant human (rh) 4-1BB, rhCD27, rhCD40, rhBAFF R, rhCD30, rhDR3, rhDR6, rhEDAR, rhFas, rhHVEM, rhGITR, rhLTR B, recominant mouse (rm) NGF R, rhOPG, rmOX40, rhRANK, rhTAJ, rhTNF RI or rhTNF RII is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Canine NGFR/TNFRSF16 Antibody

Detection of Human NGF R/TNFRSF16 antibody by Western Blot.

Detection of Human NGF R/TNFRSF16 by Western Blot.

Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NGF R/TNFRSF16 at approximately 65 kDa (as indicated). GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of NGF R/TNFRSF16 antibody in SH-SY5Y Human Cell Line antibody by Flow Cytometry.

Detection of NGF R/TNFRSF16 in SH‑SY5Y Human Cell Line by Flow Cytometry.

SH-SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
NGF R/TNFRSF16 antibody in Canine Mesenchymal Stem Cells by Immunocytochemistry (ICC).

NGF R/TNFRSF16 in Canine Mesenchymal Stem Cells.

NGF R/TNFRSF16 was detected in immersion fixed canine mesenchymal stem cells using Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
NGF R/TNFRSF16 antibody in Human Mesenchymal Stem Cells by Immunocytochemistry (ICC).

NGF R/TNFRSF16 in Human Mesenchymal Stem Cells.

NGF R/TNFRSF16 was detected in immersion fixed human mesenchymal stem cells using Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
NGF R/TNFRSF16 antibody in Human Brain by Immunohistochemistry (IHC-P).

NGF R/TNFRSF16 in Human Brain.

NGF R/TNFRSF16 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 25 µg/mL Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of NGFR/TNFRSF16 by Western Blot

Detection of NGFR/TNFRSF16 by Western Blot

HPV16 E6-E7 increased p75NTR expression through PI3K/Akt signaling pathway in TE-1 cellsA. Proteins involved in PI3K/Akt signaling pathway and p75NTR protein were analysed by western blotting. TE-1 cells were treated by negative control RNA, GAPDH RNA, PI3K siRNA, respectively. Equal protein loading was evaluated by beta -actin. “-” means HPV16 E6-E7 negative which represents TE-1-control cells, “+” means HPV16 E6-E7 positive which represents TE-1-psb cells. B. Proteins involved in PI3K/Akt signaling pathway and p75NTR protein were analysed by western blotting. TE-1 cells were treated by negative control RNA, GAPDH RNA, Akt siRNA, respectively. Equal protein loading was evaluated by beta -actin. “-” means HPV16 E6-E7 negative which represents TE-1-control cells, “+” means HPV16 E6-E7 positive which represents TE-1-psb cells. C. Densitometric of western blotting bands in Figure A were analyzed and expressed relative to the loading control, beta -actin. Data are typical of three experiments and the histogram values are mean ± S.D. *P<0.05,**P<0.01,***P<0.001, relative to control cells in control siRNA group. ###P<0.001, relative to TE-1-psb cell in control siRNA group. D. Densitometric of western blotting bands in Figure B were analyzed and expressed relative to the loading control, beta -actin. Data are typical of three experiments and the histogram values are mean ± S.D. *P<0.05,**P<0.01,***P<0.001, relative to control cells in control siRNA group or Akt siRNA group. ###P<0.001, relative to TE-1-psb cell in control siRNA group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27489353), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of NGFR/TNFRSF16 by Western Blot

Detection of NGFR/TNFRSF16 by Western Blot

HPV16 E6-E7 increased p75NTR expression through PI3K/Akt signaling pathway in Eca109 cellsA. Proteins involved in PI3K/Akt signaling pathway and p75NTR protein were analysed by western blotting. Eca109 cells were treated by negative control RNA, GAPDH RNA, PI3K siRNA, respectively. Equal protein loading was evaluated by beta -actin. “-” means HPV16 E6-E7 negative which represents Eca109-control cells, “+”means HPV16 E6-E7 positive which represents Eca109-psb cells. B. Proteins involved in PI3K/Akt signaling pathway and p75NTR protein were analysed by western blotting. Eca109 cells were treated by negative control RNA, GAPDH RNA, Akt siRNA, respectively. Equal protein loading was evaluated by beta -actin. “-”means HPV16 E6-E7 negative which represents Eca109-control cells, “+”means HPV16 E6-E7 positive which represents Eca109-psb cells. C. Densitometric of western blotting bands in Figure A were analyzed and expressed relative to the loading control, beta -actin. Data are typical of three experiments and the histogram values are mean ± S.D. *P<0.05,**P<0.01,***P<0.001, relative to control cells in control siRNA group. ###P<0.001, relative to Eca109-psb cell in control siRNA group. D. Densitometric of western blotting bands in Figure B were analyzed and expressed relative to the loading control, beta -actin. Data are typical of three experiments and the histogram values are mean ± S.D. *P<0.05,**P<0.01,***P<0.001, relative to control cells in control siRNA group or Akt siRNA group. ###P<0.001, relative to Eca109-psb cell in control siRNA group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27489353), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of NGFR/TNFRSF16 by Western Blot

Detection of NGFR/TNFRSF16 by Western Blot

HPV16 E6-E7 increased p75NTR expression through PI3K/Akt signaling pathway in TE-1 cellsA. Proteins involved in PI3K/Akt signaling pathway and p75NTR protein were analysed by western blotting. TE-1 cells were treated by negative control RNA, GAPDH RNA, PI3K siRNA, respectively. Equal protein loading was evaluated by beta -actin. “-” means HPV16 E6-E7 negative which represents TE-1-control cells, “+” means HPV16 E6-E7 positive which represents TE-1-psb cells. B. Proteins involved in PI3K/Akt signaling pathway and p75NTR protein were analysed by western blotting. TE-1 cells were treated by negative control RNA, GAPDH RNA, Akt siRNA, respectively. Equal protein loading was evaluated by beta -actin. “-” means HPV16 E6-E7 negative which represents TE-1-control cells, “+” means HPV16 E6-E7 positive which represents TE-1-psb cells. C. Densitometric of western blotting bands in Figure A were analyzed and expressed relative to the loading control, beta -actin. Data are typical of three experiments and the histogram values are mean ± S.D. *P<0.05,**P<0.01,***P<0.001, relative to control cells in control siRNA group. ###P<0.001, relative to TE-1-psb cell in control siRNA group. D. Densitometric of western blotting bands in Figure B were analyzed and expressed relative to the loading control, beta -actin. Data are typical of three experiments and the histogram values are mean ± S.D. *P<0.05,**P<0.01,***P<0.001, relative to control cells in control siRNA group or Akt siRNA group. ###P<0.001, relative to TE-1-psb cell in control siRNA group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27489353), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of NGFR/TNFRSF16 by Western Blot

Detection of NGFR/TNFRSF16 by Western Blot

PI3K/Akt signaling pathway activation and increased p75NTR expression induced by HPV16 E6-E7 were inhibited by LY294002 in ESCC cellsA. Proteins involved in PI3K/Akt signaling pathway and p75NTR protein were analysed by western blotting. Cells were treated with LY294002 (10μmol/L) for 24h. Equal protein loading was evaluated by beta -actin. B. Densitometric of western blotting bands in Figure A were analyzed and expressed relative to the loading control, beta -actin. Data are typical of three experiments and the histogram values are mean ± S.D. **P<0.01,***P<0.001, relative to control cells without LY294002 treatment. ###P<0.001, relative to psb cells without LY294002 treatment. C. p-Akt, PI3K and p75NTR proteins of ESCC cells were detected by immunofluorescence. D. PI3K and p75NTR proteins of spheres formed from ESCC cells were detected by immunofluorescence. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27489353), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of NGFR/TNFRSF16 by Immunohistochemistry

Detection of NGFR/TNFRSF16 by Immunohistochemistry

Visualization of CD271 and CD81 expression in bone marrow vascular regions by confocal microscopy.(A) Confocal scan of vascular region in BM biopsies with 3D orthographic cross-section view, co-stained with mouse anti-CD271, rabbit anti-CD81, and DAPI. (B) Single channel data for the florescent markers in A. (C) Intensity profile for all channels in A across a cell of interest in a representative z plane. Scale bars represent 50 μm. Yellow dashed lines indicated vessel surface. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36876630), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of NGFR/TNFRSF16 by Immunohistochemistry

Detection of NGFR/TNFRSF16 by Immunohistochemistry

Visualization of CD271 and CD81 expression in bone marrow vascular regions by confocal microscopy.(A) Confocal scan of vascular region in BM biopsies with 3D orthographic cross-section view, co-stained with mouse anti-CD271, rabbit anti-CD81, and DAPI. (B) Single channel data for the florescent markers in A. (C) Intensity profile for all channels in A across a cell of interest in a representative z plane. Scale bars represent 50 μm. Yellow dashed lines indicated vessel surface. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36876630), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Canine NGFR/TNFRSF16 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: SHSY-5Y human cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed canine and human mesenchymal stem cells

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Western Blot

2 µg/mL
Sample: SW480 human colorectal adenocarcinoma cell line
under non-reducing conditions only

Reviewed Applications

Read 3 reviews rated 4.7 using MAB367 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NGFR/TNFRSF16

NGF R is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family (1) and has been designated TNFRSF16. This receptor is also known as p75 NTR (neurotrophin receptor) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including Brain-Derived Neurotrophic Factor (BDNF), Neurotrophin-3 and Neurotrophin-4/5. NGF R is a 75 kDa protein that is expressed in neuronal axons, Schwann’s cells and perineural cells of peripheral nerves (1). Neural crest stem cells have been isolated based on their surface expression of NGF R (2, 3). In addition, neuroepithelial-derived NGF R positive cells have also been demonstrated to be able to differentiate into neurons, smooth muscle and Schwann cells in culture (4). NGF R has been used as a marker to identify mesenchymal precursors as well as hepatic stellate cells (5, 6).

References

  1. Barker, P.A. et al. (1992) Mol. Cell Biochem. 110:1.
  2. Stemple, D.L. et al. (1992) Cell 71:973.
  3. Morrison, S.J. et al. (1999) Cell 96:737.
  4. Mujtaba, T. et al. (1998) Dev. Biol. 200:1.
  5. Campagnolo, L. et al. (2001) Biol. Reprod. 64:464.
  6. Cassiman, D. et al. (2001) Hepatology 33:148.

    Long Name

    Nerve Growth Factor Receptor

    Alternate Names

    CD271, NGF R, p75 NTR, TNFRSF16

    Entrez Gene IDs

    4804 (Human); 18053 (Mouse); 117089 (Rat)

    Gene Symbol

    NGFR

    UniProt

    P08138

    Additional NGFR/TNFRSF16 Products

    Product Documents for Human/Canine NGFR/TNFRSF16 Antibody

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    Product Specific Notices for Human/Canine NGFR/TNFRSF16 Antibody

    For research use only

    Citations for Human/Canine NGFR/TNFRSF16 Antibody

    Customer Reviews for Human/Canine NGFR/TNFRSF16 Antibody (3)

    4.7 out of 5
    3 Customer Ratings
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    Customer Images

    Showing  1 - 3 of 3 reviews Showing All
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    • Human/Canine NGFR/TNFRSF16 Antibody
      Name: Anonymous
      Application: Immunocytochemistry
      Sample Tested: primary astrocytes
      Species: Human
      Verified Customer | Posted 02/25/2022
      Primary astrocytes co-stained for cilia (Arl13b, red, top left), P75NTR (green, bottom left) and DAPI (blue, top right) and merged image (bottom right).
      Human/Canine NGFR/TNFRSF16 Antibody MAB367
    • Human/Canine NGFR/TNFRSF16 Antibody
      Name: Anonymous
      Application: Immunohistochemistry
      Sample Tested: Uterus tissue
      Species: Human
      Verified Customer | Posted 09/04/2021
      Human/Canine NGFR/TNFRSF16 Antibody MAB367
    • Human/Canine NGFR/TNFRSF16 Antibody
      Name: Christian Schuerch
      Application: Immunohistochemistry-Frozen
      Sample Tested: human tonsil
      Species: Human
      Verified Customer | Posted 02/29/2020
      NGFR on human tonsil, dilution 1:100
      pH 9 epitope retrieval
      Human/Canine NGFR/TNFRSF16 Antibody MAB367

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