Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, Immunoprecipitation, CyTOF-ready

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Carboxypeptidase E/CPE Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase E/CPE
Arg42-Ser453
Accession # P16870

Specificity

Detects human Carboxypeptidase E/CPE in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human CPM is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Carboxypeptidase E/CPE Antibody

Carboxypeptidase E/CPE antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

Carboxypeptidase E/CPE in HepG2 Human Cell Line.

Carboxypeptidase E/CPE was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Carboxypeptidase E/CPE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3587) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Carboxypeptidase E/CPE antibody in A172 Human Cell Line antibody by Flow Cytometry.

Detection of Carboxypeptidase E/CPE in A172 Human Cell Line by Flow Cytometry.

A172 human glioblastoma cell line was stained with Goat Anti-Human Carboxypeptidase E/CPE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3587, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol and saponin.

Carboxypeptidase E/CPE antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Carboxypeptidase E/CPE in Mouse Embryo.

Carboxypeptidase E/CPE was detected in immersion fixed frozen sections of mouse embryo (9.5 d.p.c.) using Goat Anti-Human Carboxypeptidase E/CPE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3587) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the developing liver. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Carboxypeptidase E/CPE by Western Blot

Detection of Carboxypeptidase E/CPE by Western Blot

SG proteins are reduced in human iN with PWS deletion.(A) Transcript levels of MAP2 and DCX are significantly increased in control and PWS iN at 14 days postinduction. PWS iN are normalized to averaged control iN. Each data point represents 1 induction experiment (n = 3), plotted with mean ± SD and analyzed by 1-way ANOVA. (B) Transcript levels of MAGEL2 are significantly increased in control iN at 14 days postinduction. Each data point represents 1 induction experiment (n = 3), plotted as mean ± SD and analyzed by 1-way ANOVA. (C) Western blot analysis confirms reduced expression of PCSK1, PCSK2, CHGB, and CPE in PWS iN at 14 days postinduction. GAPDH served as loading control. (D) Quantification of Western blot analysis showed reduced expression of PCSK1, PCSK2, CHGB, and CPE in PWS iN. Each target protein is first normalized to GAPDH, and then PWS iN is normalized to averaged control iN. Each data point represents 1 induction experiment (n = 4), plotted as mean ± SD and analyzed by 1-way ANOVA; *P < 0.05. (E) Western blot analysis confirms reduced expression of PCSK1, PCSK2, CHGB, and CPE in PWS iN compared with control iN at 3, 7, 10, and 14 days postinduction. GAPDH served as loading control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32879135), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Carboxypeptidase E/CPE Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (9.5 d.p.c.)

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase E/CPE (Catalog # 3587-ZN), see our available Western blot detection antibodies

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

0.1 µg/mL
Sample: Recombinant Human Carboxypeptidase E/CPE (Catalog # 3587-ZN)

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carboxypeptidase E/CPE

Encoded by the CPE gene and also known as Carboxypeptidase H, CPE is a single chain peptidase with an optimal pH range between 5.0‑6.0. It is a zinc metallocarboxypeptidase that removes basic amino acids from the C-terminus of peptides (1). Like other metallocarboxypeptidases, its activity is stimulated by millimolar concentrations of Co2+. Its activity is regulated by pH-induced aggregation above pH 6.0. Its major function seems to process numerous peptide hormones and neurotransmitters. In addition to its proteolytic function, it also plays a role as a sorting receptor (2), which may be attributed to the sorting of this protein into the secretory pathway. The C-terminal domain of CPE causes the peripheral association of CPE with membranes below neutral pH, resulting in the association of this protein into membranes (3). CPE knockout mice live but become obese due to impaired glucose clearance and insulin resistance (4).

References

  1. Fricker, L.D. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 840.
  2. Cool, D.R. et al. (1997) Cell 88:73.
  3. Zhang, C-F. et al.  (2003) Biochem. J. 369:453.
  4. Cawley, N.X. et al. (2004) Endocrinology 145:5807.

Alternate Names

CPE, CPH-1

Entrez Gene IDs

1363 (Human); 12876 (Mouse); 25669 (Rat)

Gene Symbol

CPE

UniProt

P16870

Additional Carboxypeptidase E/CPE Products

Product Documents for Human Carboxypeptidase E/CPE Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Carboxypeptidase E/CPE Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators
Obesity

Citations for Human Carboxypeptidase E/CPE Antibody

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Protocols

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