Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Immunohistochemistry, Western Blot, Simple Western

Cited:

Western Blot, Immunocytochemistry, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Caveolin-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Caveolin-1
Ser2-Ser104
Accession # Q03135

Specificity

Detects human Caveolin-1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Caveolin‑1 Antibody

Detection of Human Caveolin-1 antibody by Western Blot.

Detection of Human Caveolin‑1 by Western Blot.

Western blot shows lysates of HUVEC human umbilical vein endothelial cells, A431 human epithelial carcinoma cell line, and A549 human lung carcinoma cell line. PVDF Membrane was probed with 0.2 µg/mL of Goat Anti-Human Caveolin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5736) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). Specific bands were detected for Caveolin-1 at approximately 21 to 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Caveolin-1 antibody in Human Liver by Immunohistochemistry (IHC-P).

Caveolin‑1 in Human Liver.

Caveolin-1 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Caveolin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5736) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; (CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in bile canaliculi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Caveolin‑1 antibody by Simple WesternTM.

Detection of Human Caveolin‑1 by Simple WesternTM.

Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells and A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Caveolin-1 at approximately 29 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Caveolin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5736) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human Caveolin-1 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human Caveolin‑1 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Caveolin-1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Caveolin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5736) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Caveolin-1 at approximately 25 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Shows Caveolin‑1 Specificity Using Knockout Cell Line.

Western Blot Shows Caveolin‑1 Specificity Using Knockout Cell Line.

Western blot shows lysates of U‑87 MG human glioblastoma/astrocytoma parental cell line and Caveolin‑1 knockout U-87 MG cell line (KO). Nitrocellulose membrane was probed with Goat Anti-Human Caveolin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5736) followed by HRP-conjugated secondary antibody. A specific band was detected for Caveolin‑1 at approximately 20.4 kDa (as indicated) in the parental U-87 MG cell line, but is not detectable in knockout U-87 MG cell line. Primary antibody dilution used: 0.5 µg/mL. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Applications for Human Caveolin‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver  

Knockout Validated

Caveolin‑1 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Caveolin‑1 knockout HeLa cell line.

Simple Western

2 µg/mL
Sample: HUVEC human umbilical vein endothelial cells and A431 human epithelial carcinoma cell line

Western Blot

0.2 µg/mL
Sample: HUVEC human umbilical vein endothelial cells, A431 human epithelial carcinoma cell line, and A549 human lung carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Caveolin-1

Caveolin-1 is a palmitoylated 22 kDa membrane-associated protein in caveolae which are cholesterol-rich invaginations in the plasma membrane involved in vesicular transport and the regulation of lipid rafts and signal transduction. Caveolin-1 expression is dysregulated during cancer progression and exhibits both positive and negative effects on tumor progression. The central region of Caveolin-1 (aa 105 - 125) is buried in the lipid layer, while the N- and C-terminal flanking regions are exposed to the cytoplasm and interact with many other proteins. Alternate splicing in human, mouse, and rat Caveolin-1 generates an isoform with a deletion of the
N-terminal 31 residues.

Alternate Names

CAV1, Caveolin1, MSTP085, VIP21

Entrez Gene IDs

857 (Human); 12389 (Mouse); 25404 (Rat)

Gene Symbol

CAV1

UniProt

Q03135

Additional Caveolin-1 Products

Product Documents for Human Caveolin‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Caveolin‑1 Antibody

For research use only

Citations for Human Caveolin‑1 Antibody

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Protocols

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