Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 23002
View all CCL2/JE/MCP-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL2/MCP-1
Gln24-Thr99
Accession # P13500

Specificity

Detects human CCL2/JE/MCP-1 in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human CCL1, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9/10/MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CCL2/JE/MCP‑1 Antibody

CCL2/JE/MCP-1 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

CCL2/JE/MCP‑1 in Human Breast Cancer Tissue.

CCL2/JE/MCP-1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB2791) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human CCL2/JE/MCP‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Reviewed Applications

Read 1 review rated 5 using MAB2791 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL2/JE/MCP-1

MCP-1 is a beta -chemokine that has been designated CCL2. It is produced by a variety of cell types and is chemotactic for monocytes.

Alternate Names

GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF

Entrez Gene IDs

6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)

Gene Symbol

CCL2

UniProt

P13500

Additional CCL2/JE/MCP-1 Products

Product Documents for Human CCL2/JE/MCP‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL2/JE/MCP‑1 Antibody

For research use only

Citations for Human CCL2/JE/MCP‑1 Antibody

Customer Reviews for Human CCL2/JE/MCP‑1 Antibody (1)

5 out of 5
1 Customer Rating
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  • Human CCL2/JE/MCP-1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: synovium
    Species: Human
    Verified Customer | Posted 09/02/2021
    Human CCL2/JE/MCP‑1 Antibody MAB2791

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Protocols

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FAQs

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