Human CCL27/CTACK Antibody

Catalog # Availability Size / Price Qty
AF376
AF376-SP
Human CCL27/CTACK Antibody in Data
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (6)

Human CCL27/CTACK Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL27/CTACK in direct ELISAs and Western blots. In Western blots, less than 2% cross-reactivity with recombinant mouse CCL27/CTACK is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CCL27/CTACK (R&D Systems, Catalog # 376-CT)
Phe25-Gly112
Accession # Q9Y4X3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CCL27/CTACK (Catalog # 376-CT)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization

Measured by its ability to neutralize CCL27/CTACK-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR10/GPR2. At 55 μg/mL, this antibody will neutralize > 70% of the chemotactic effect due to 1 μg/mL Recombinant Human CCL27/CTACK.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Neutralization Chemotaxis Induced by CCL27/CTACK and Neutralization by Human CCL27/CTACK Antibody. View Larger

Chemotaxis Induced by CCL27/CTACK and Neutralization by Human CCL27/CTACK Antibody. Recombinant Human CCL27/CTACK (Catalog # 376-CT) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR10/GPR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL27/CTACK (1 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL27/CTACK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF376). At 55 µg/mL, this antibody will neutralize > 70% of the chemotactic effect.

Immunohistochemistry CCL27/CTACK in Human Skin. View Larger

CCL27/CTACK in Human Skin. CCL27/CTACK was detected in immersion fixed frozen sections of human skin using Human CCL27/CTACK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF376) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hemotoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL27/CTACK

CCL27, also known as CTACK (cutaneous T cell-attracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines. Mature human CCL27 is an 88 amino acid (aa) protein that shares 57% aa sequence identity with mouse and rat CCL27. It shares 11-35% aa sequence identity with other human CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration. CCL27 is constitutively expressed by keratinocytes and is upregulated by inflammatory stimuli and in wounded skin. CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL-1, and determinants on the surface of fibroblasts and endothelial cells. CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to the skin during inflammation. Endothelial cell-bound CCL27 can mediate the adhesion of those cells to CLA+ T cells. CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing. In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides.

Entrez Gene IDs
10850 (Human); 20301 (Mouse); 362505 (Rat)
Alternate Names
ALP; C-C motif chemokine 27; CCL27; chemokine (C-C motif) ligand 27; CTACK; CTACKSmall-inducible cytokine A27; CTAK; cutaneous T-cell attracting chemokine; Cutaneous T-cell-attracting chemokine; ESKINE; IL-11 Ralpha-locus chemokine; ILC; ILCCC chemokine ILC; PESKY; SCYA27IL-11 R-alpha-locus chemokine; skinkine; small inducible cytokine subfamily A (Cys-Cys), member 27

Product Datasheets

Citations for Human CCL27/CTACK Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Chemokine CCL27 is a novel plasma biomarker for identification the nasopharyngeal carcinoma patients from the Epstein-Barr virus capsid antigen-specific IgA seropositive population
    Authors: MJ Mao, N Xue, XP Wang, PD Chi, YJ Liu, Q Huang, SQ Dai, WL Liu
    BMC Cancer, 2018;18(1):9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Gene expression signatures for tumor progression, tumor subtype, and tumor thickness in laser-microdissected melanoma tissues.
    Authors: Jaeger J, Koczan D, Thiesen HJ, Ibrahim SM, Gross G, Spang R, Kunz M
    Clin. Cancer Res., 2007;13(3):806-15.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human CCL27/CTACK Antibody

Average Rating: 4.2 (Based on 6 Reviews)

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Human CCL27/CTACK Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human CCL27/CTACK Antibody
By Anonymous on 09/05/2019
Application: ELISA Sample Tested: Serum Species: Human

Human CCL27/CTACK Antibody
By Anonymous on 11/08/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human,Mouse

Human CCL27/CTACK Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human CCL27/CTACK Antibody
By Anonymous on 11/02/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Human CCL27/CTACK Antibody
By Anonymous on 11/15/2017
Application: ELISA Sample Tested: Plasma Species: Human

MAB376 was used in a sandwich ELISA with biotinylated AF376 as detection at a concentration of 50ng/ml.