Human CD11c Antibody Summary
Accession # P20702
Complete Your Research
Complete Your Experiment
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human CD11c by Western Blot. Western blot shows lysates of human mature myeloid dendritic cells. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human CD11c Monoclonal Antibody (Catalog # MAB17771) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CD11c at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1with 0.05% Tween® 20 in the blotting buffer.
Detection of CD11c in human tonsil. CD11c was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD11c Monoclonal Antibody (Catalog # MAB17771) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD11c, also known as Integrin alpha X, is an approximately 150 kDa type I transmembrane glycoprotein that heterodimerizes with Integrin beta 2, also known as CD18. The CD11c/CD18 complex, also called CR4 (complement receptor type 4) is expressed on macrophages, dendritic cells and hairy cell leukemias, with lower amounts on other myeloid cells and activated B, NK and some cytotoxic T cells. It binds fibrinogen and has been reported to be a receptor for complement component iC3b (1‑3). The human CD11c extracellular domain (amino acids 20-1107) shares 70 - 76% amino acid sequence identity with mouse, rat and canine CD11c.
- Hogg, N. et al. (1986) Eur. J. Immunol. 16(3):240.
- Knapp, W.B. et al. eds. (1989) Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York.
- Stacker, S.A. and T.A. Springer, J. Immunol. (1991) 146(2):648.
Citation for Human CD11c Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
An Allosteric Shift in CD11c Affinity Activates a Proatherogenic State in Arrested Intermediate Monocytes
Authors: AA Hernandez, GA Foster, SR Soderberg, A Fernandez, MB Reynolds, MK Orser, KA Bailey, JH Rogers, GD Singh, H Wu, AG Passerini, SI Simon
J Immunol, 2020;0(0):.
Sample Types: Cell Culture Supernates
Applications: Western Blot
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human CD11c Antibody
There are currently no reviews for this product. Be the first to review Human CD11c Antibody and earn rewards!
Have you used Human CD11c Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image