Human CD11c Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD11c in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Integrin  alpha 1, 2, 4, 5, 6, 8, 9, 10, 11, L, M, rhIntegrin alpha 2b/ beta 3, alpha 3/ beta 1, alpha 6X1/ beta 4, alpha 7X2/ beta 1, alpha D/ beta 2, alpha E/ beta 7, alpha V/ beta 6, recombinant mouse Integrin alpha X, and alpha 7X2 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 538207
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human CD11c
    aa 20-1107
    Accession # P20702
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human CD11c by Western Blot.

Western blot shows lysates of human mature myeloid dendritic cells. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human CD11c Monoclonal Antibody (Catalog # MAB17771) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CD11c at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 with 0.05% Tween® 20 in the blotting buffer.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD11c

CD11c, also known as Integrin alpha X, is an approximately 150 kDa type I transmembrane glycoprotein that heterodimerizes with Integrin beta 2, also known as CD18. The CD11c/CD18 complex, also called CR4 (complement receptor type 4) is expressed on macrophages, dendritic cells and hairy cell leukemias, with lower amounts on other myeloid cells and activated B, NK and some cytotoxic T cells. It binds fibrinogen and has been reported to be a receptor for complement component iC3b (1‑3). The human CD11c extracellular domain (amino acids 20-1107) shares 70 - 76% amino acid sequence identity with mouse, rat and canine CD11c.

  • References:
    1. Hogg, N. et al. (1986) Eur. J. Immunol. 16(3):240.
    2. Knapp, W.B. et al. eds. (1989) Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York.
    3. Stacker, S.A. and T.A. Springer, J. Immunol. (1991) 146(2):648.
  • Entrez Gene IDs:
    3687 (Human); 16411 (Mouse); 64350 (Rat)
  • Alternate Names:
    95 alpha chain; 95; 95, alpha subunit; CD11 antigen-like family member C; CD11c antigen; CD11c; CD11Cleu M5, alpha subunit; Integrin alpha X; integrin alpha-X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); ITGAX; Leu M5; Leukocyte adhesion glycoprotein p150; Leukocyte adhesion receptor p150; leukocyte surface antigen p150; myeloid membrane antigen, alpha subunit; p150 95 integrin alpha chain; p150,95 alpha; SLEB6
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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