Human CD14 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB3832R-100
MAB3832R-SP
Detection of CD14 in Human PBMCs by Flow Cytometry.
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Human CD14 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD14 in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 134620R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD14
Thr20-Cys352
Accession # P08571
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize lipopolysacharide (LPS)-induced TNF‑ alpha secretion in the THP-1 human acute monocytic leukemia cell line. At 10 µg/mL, this anti-human CD14 antibody will neutralize >60% of 0.5 ng/mL LPS-induced TNF-alpha secretion.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of CD14 in Human PBMCs by Flow Cytometry. View Larger

Detection of CD14 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 Monoclonal Antibody (Catalog # MAB3832R) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Neutralization TNF-alpha  Secretion Induced by LPS and Neutralization by Human CD14 Antibody. View Larger

TNF-alpha Secretion Induced by LPS and Neutralization by Human CD14 Antibody. Lipopolysaccharide (LPS) stimulates TNF-alpha secretion in the THP-1 human acute monocytic leukemia cell line in a dose-dependent manner (orange line). TNF-alpha secre­tion elicited by LPS is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD14 Monoclonal Antibody (Catalog # MAB3832R). At 10 μg/mL, this anti-human CD14 antibody will neutralize 60% of 0.5 ng/mL LPS-induced TNF-alpha secretion.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD14

CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) residue precursor protein with a 19 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.

References
  1. Wright, S.D. et al. (1990) Science 249:1431.
  2. Pugin, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744.
  3. Beutler, B. (2000) Current Opinion in Immunology 12:20.
  4. Stelter, F. (2000) Chem. Immunol. 74:25.
Entrez Gene IDs
929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)
Alternate Names
CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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