Human CD161 Antibody

Catalog # Availability Size / Price Qty
MAB7448
MAB7448-SP
Detection of Human CD161 in Human Lymphocytes
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human CD161 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD161 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 702228
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human CD161
Accession # Q12918
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Human CD161 antibody in Human Lymphocytes View Larger

Detection of Human CD161 in Human Lymphocytes Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse IgG2B Isotype control (Catalog # MAB0041) or (B) Mouse Anti-Human CD161 Monoclonal Antibody (Catalog # MAB7448) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD161

CD161, also known as NKR-P1A and KLRB1, is a 40 kDa type II transmembrane glycoprotein that contains one C-type lectin domain in its extracellular region. CD161 is expressed as a disulfide-linked dimer on the surface of Th17 cells and NK cells as well as on subsets of CD1-restricted T cells, intestinal NT cells, peripheral memory T cells, monocytes, and dendritic cells. It binds to OCIL/CLEC2d, leading to an inhibition of NK cell-mediated cytotoxicity and IFN-g secretion. Alternatively, CD161 can enhance TCR activation to CD1d ligation. CD161+ cell populations are depleted in ulcerative colitis, Grave’s disease, and AIDS, although CD161+ T cells are activated during asthmatic attacks. Additional related proteins are expressed in mouse but not human: the inhibitory NKR‑P1B and D, and the stimulatory NKR‑P1A, C, and F. Human CD161 shares 47% aa sequence identity with mouse and rat NKR-P1A.

Entrez Gene IDs
3820 (Human); 25192 (Rat)
Alternate Names
CD161 antigen; CD161; CLEC5B; CLEC5BC-type lectin domain family 5 member B; HNKR-P1a; killer cell lectin-like receptor subfamily B member 1; killer cell lectin-like receptor subfamily B, member 1; KLRB1; Ly59; Natural killer cell surface protein P1A; NKR; NKRP1; NKR-P1; NKRP1A; NKR-P1AMGC138614; NKRP1ANKR

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human CD161 Antibody

There are currently no reviews for this product. Be the first to review Human CD161 Antibody and earn rewards!

Have you used Human CD161 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review