Key Product Details

Species Reactivity

Human

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1069433
View all CD45RA Primary Antibodies »

Product Specifications

Immunogen

Whole cells (naïve T cells)
Accession # P08575

Specificity

Detects CD45RA in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CD45RA Antibody

Detection of CD45RA in Human Appendix via Multiplex Immunofluorescence staining on COMET™

CD45RA was detected in immersion fixed paraffin-embedded sections of human appendix using Mouse Anti-Human CD45RA Monoclonal Antibody (MAB11444) at 2.5µg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100).Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of CD45RA in PBMC lymphocytes by Flow Cytometry.

PBMC lymphocytes were stained with either (A) Mouse Anti-Human CD45RA Monoclonal Antibody (Catalog # MAB11444) or (B) Mouse IgG2A Isotype Control (MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B) and followed with Mouse Anti-Human CD45RO Alexa Fluor® 488-conjugated Monoclonal Antibody (FAB10642G). View our protocol for Staining Membrane-associated Proteins.

Detection of CD45RA in Human Tonsil.

CD45RA was detected in immersion fixed paraffin-embedded sections of Human Tonsil using Mouse Anti-Human CD45RA Monoclonal Antibody (Catalog # MAB11444) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human CD45RA Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: PBMC lymphocytes

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Tonsil

Multiplex Immunofluorescence

2.5 µg/mL
Sample: Immersion fixed paraffin embedded sections of human appendix

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD45RA

This monoclonal antibody specifically binds to the 220 kDa isoform of human CD45, CD45RA.  CD45RA is a single chain type I glycoprotein that is encoded by the human PTPRC gene.  In humans, CD45RA is one of six isoforms of the CD45 complex.  These different isoforms are the result of alternative splicing and some of the other isoforms include CD45RO, CD45RB, and CD45RC.  CD45RO, CD45RB, CD45RC, and CD45RA are primarily expressed on T cells.  CD45RA contains exon 4 but lacks both exons 5 and 6.  CD45RO contains exons 3, 7, and 8 but not RA, RB, and RC exons.  Phenotypically, CD45RA is highly expressed on naïve T cells whereas CD45RO is expressed on activated and memory T cells.  Other cell types such as activated monocytes and B cells can also express CD45RO.  Both CD45RA and CD45RO are used to characterize different subsets of Memory T cells.

Long Name

CD45R, A isoform

Alternate Names

B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Entrez Gene IDs

5788 (Human)

Gene Symbol

PTPRC

UniProt

P08575

Additional CD45RA Products

Product Documents for Human CD45RA Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD45RA Antibody

For research use only

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Protocols

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