Human CD58/LFA-3 Antibody

Catalog # Availability Size / Price Qty
AF1689
AF1689-SP
Human CD58/LFA-3 Antibody in Immunohistochemistry
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Product Details
Citations (1)
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Human CD58/LFA-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD58/LFA‑3 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD58/LFA‑3
Phe29-Arg215
Accession # P19256
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CD58/LFA‑3 (Catalog # 1689-CD)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize CD58/LFA‑3-induced proliferation in the human CD3+ T cells. Bierer, B.E. et al. (1988) J. Immunol. 140:3358. The Neutralization Dose (ND50) is typically 3-12 µg/mL in the presence of 0.2 µg/well Recombinant Human CD58/LFA‑3 and 2 ng/well Mouse Anti-Human CD3 Monoclonal Antibody OKT3.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry CD58/LFA‑3 in Human Tonsil. View Larger

CD58/LFA‑3 in Human Tonsil. CD58/LFA‑3 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Goat Anti-Human CD58/LFA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1689) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of lymphocytes in lymphatic nodules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization Cell Proliferation Induced by CD58/LFA‑3 and Neutral­ization by Human CD58/LFA‑3 Antibody. View Larger

Cell Proliferation Induced by CD58/LFA‑3 and Neutral­ization by Human CD58/LFA‑3 Antibody. Recombinant Human CD58/LFA‑3 (Catalog # 1689‑CD) stimulates proliferation in the human CD3+ T cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human CD58/LFA‑3 (0.2 µg/well) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD58/LFA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1689). The ND50 is typically 3-12 µg/mL in the presence of Mouse Anti-Human CD3 Monoclonal Antibody OKT3 (2 ng/well).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD58/LFA-3

T cells require a signal induced by the engagement of the T cell receptor and a “co-stimulatory” signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Many cell-bound receptor-ligand pairs have now been shown to be involved in T cell co-stimulation including CD58/CD2 in humans and CD48/CD2 in mice and rats. CD58, also known as lymphocyte function-associated antigen (LFA-3), is a 210 amino acid protein that belongs to the CD2 family of the immunoglobulin superfamily (1). CD58 is widely expressed on hematopoietic and non-hematopoietic human tissue and has been found on leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts of human origin (2). No mouse or rat homolog of CD58 has as of yet been identified. CD58 has only one known ligand, CD2. CD2 is expressed on T cells, NK cells and dendritic cells (2-4). CD2 ligation by CD58 has been shown to mediate T cell adhesion, T cell activation, T cell cytokine production, and T cell and NK cells cytotoxic activity (1, 3, 5, 6). In dendritic cells, CD2 engagement increases MHC Class II, CD40, CD80, CD86, CD58, and CCR7 and induces IL-1 beta and IL-12 cytokine secretion (4).

References
  1. Davis, S.J. and P.A. van der Merwe (1996) Immunol. Today 17:177.
  2. Smith, M.E. and J.A. Thomas (1990) J. Clin. Pathol. 43:893.
  3. Bolhuis, R.L. Roozemond, R.C. and R.J. van de Griend (1986) J. Immunol. 136:3939.
  4. Crawford, K. et al. (2003) Blood 102:1745.
  5. Kanner, S.B. et al. (1992) J. Immunol. 148:2023.
  6. Bullens, M.A. et al. (2001) International Immunol. 12:181.
Entrez Gene IDs
965 (Human); 102123839 (Cynomolgus Monkey); 710732 (Rhesus Macaque)
Alternate Names
Ag3; CD58 antigen; CD58 antigen, (lymphocyte function-associated antigen 3); CD58 molecule; CD58; FLJ23181; FLJ43722; LFA3; LFA-3; LFA3ag3; lymphocyte function-associated antigen 3; Surface glycoprotein LFA-3

Product Datasheets

Citation for Human CD58/LFA-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Novel natural killer cell-mediated cancer immunotherapeutic activity of anisomycin against hepatocellular carcinoma cells
    Authors: M Kim, SJ Lee, S Shin, KS Park, SY Park, CH Lee
    Sci Rep, 2018;8(1):10668.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Immunohistochemistry Reagents

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Reconstitution Buffers

Secondary Antibodies

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