Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Western Blot, Neutralization, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CD59 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD59
Leu26-Asn102
Accession # P13987

Specificity

Detects human CD59 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CD59 Antibody

Detection of Human CD59 antibody by Western Blot.

Detection of Human CD59 by Western Blot.

Western blot shows lysates of human breast tissue and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD59 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1987) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD59 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human CD59 by Simple WesternTM.

Simple Western lane view shows lysates of Exosome Standards (HT‑29) (NBP3-11685) and HUVEC human umbilical vein endothelial cells, loaded at 0.5 mg/ml. A specific band was detected for CD59 at approximately 27-33 kDa (as indicated) using 50 µg/ml of Goat Anti-Human CD59 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1987) followed by 1:50 dilution of HRP-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # 042-206). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.

CD59 antibody in Human Breast by Immunohistochemistry (IHC-P).

CD59 in Human Breast.

CD59 was detected in immersion fixed paraffin-embedded sections of human breast using 1.7 µg/mL Goat Anti-Human CD59 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1987) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human CD59 antibody by Simple WesternTM.

Detection of Human CD59 by Simple WesternTM.

Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for CD59 at approximately 26 kDa (as indicated) using 50 µg/mL of Goat Anti-Human CD59 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1987) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human CD59 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast

Simple Western

50 µg/mL
Sample: Exosome Standards (HT-29) (Catalog # NBP3-11685) and HUVEC human umbilical vein endothelial cells

Western Blot

1 µg/mL
Sample: Human breast tissue and HUVEC human umbilical vein endothelial cells

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD59

CD59 is a GPI-anchored membrane glycoprotein that functions to inhibit formation of the complement membrane attack complex and thereby prevent cell injury. CD59 is expressed on erythrocytes and on all peripheral blood leukocytes.

Alternate Names

CD59, HRF20, MACIF, MIC11, Protectin

Entrez Gene IDs

966 (Human)

Gene Symbol

CD59

UniProt

P13987

Additional CD59 Products

Product Documents for Human CD59 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD59 Antibody

For research use only

Citations for Human CD59 Antibody

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Protocols

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