Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CD5L Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD5L
Ser20-Gly347
Accession # O43866

Specificity

Detects human CD5L in direct ELISAs and Western blots. In these formats, less than 5% cross‑reactivity with recombinant mouse CD5L and less than 1% cross-reactivity with recombinant human CD5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CD5L Antibody

CD5L in Human Spleen.

CD5L was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human CD5L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2797) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human CD5L Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen

Western Blot

0.1 µg/mL
Sample: Recombinant Human CD5L (Catalog # 2797-CL)

Reviewed Applications

Read 4 reviews rated 4.3 using AF2797 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD5L

CD5L (CD5 antigen-like), also known as Sp alpha and AIM, is a 50 kDa secreted glycoprotein that belongs to the SRCR (scavenger receptor cysteine rich) group B family of proteins. Group B proteins are distinguished by SRCR domains that are encoded by a single exon (1 - 3). The human CD5L cDNA encodes a 347 amino acid (aa) precursor that includes a 19 aa signal sequence and three SRCR domains (4, 5). Among group B proteins, CD5L is most closely related to CD5 and CD6, with which it shares 18% and 31% aa sequence identity, respectively. CD5L is up-regulated in macrophages at inflammatory sites. It sustains inflammatory reactions by both increasing the phagocytic capacity of macrophages and impeding the apoptosis of local macrophages, NK cells, and T cells (6, 7). Agonists of the LXR and RXR nuclear hormone receptors induce CD5L upregulation in macrophages and reduce macrophage apoptosis (8, 9). Oxidized LDL (which acts through LXR/RXR) is taken up by macrophages, promoting their development into foam cells. The increased level of CD5L protects foam cells from apoptosis but permits more rapid cellular accumulation and atherosclerotic plaque formation (9). In activated B cells, however, the combination of CD5L and TGF-beta inhibits proliferation. The binding of CD5L to splenic B cells is increased following TGF-beta exposure, suggesting that TGF-beta increases the expression or availability of an unidentified CD5L receptor (5, 10). CD5L also functions as a pattern recognition molecule by binding both lipoteichoic acid on Gram positive and lipopolysaccharide on Gram negative bacteria (11). In the thymic cortex, CD5L protects cortical CD4+CD8+ thymocytes from apoptosis (12). CD5L circulates in the serum in complex with IgM (13).

References

  1. Sarrias, M.R. et al. (2004) Crit. Rev. Immunol. 24:1.
  2. Resnick, D. et al. (1994) Trends Biochem. Sci. 19:5.
  3. Mukhopadhyay, S. and S. Gordon (2004) Immunobiology 209:39.
  4. Gebe, J.A. et al. (1997) J. Biol. Chem. 272:6151.
  5. Gebe, J.A. et al. (2000) Immunol. 99:78.
  6. Haruta, I. et al. (2001) J. Biol. Chem. 276:22910.
  7. Kuwata, K. et al. (2003) Am. J. Pathol. 162:837.
  8. Valledor, A.F. et al. (2004) Proc. Natl. Acad. Sci. 101:17813.
  9. Arai, S. et al. (2005) Cell Metab. 1:201.
  10. Yusa, S. et al. (1999) Eur. J. Immunol. 29:1086.
  11. Sarrias, M-R. et al. (2005) J. Biol. Chem. 280:35391.
  12. Miyazaki, T. et al. (1999) J. Exp. Med. 189:413.
  13. Tissot, J.D. et al. (2002) Electrophoresis 23:1203.

Long Name

CD5 Antigen-like

Alternate Names

AIM, API6, CD5L, CT-2, SP-alpha

Entrez Gene IDs

922 (Human); 11801 (Mouse)

Gene Symbol

CD5L

UniProt

O43866

Additional CD5L Products

Product Documents for Human CD5L Antibody

Certificate of Analysis

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Product Specific Notices for Human CD5L Antibody

For research use only

Citations for Human CD5L Antibody

Customer Reviews for Human CD5L Antibody (4)

4.3 out of 5
4 Customer Ratings
5 Stars
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4 Stars
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3 Stars
25%
2 Stars
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1 Stars
0%

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  • Human CD5L Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human CD5L Antibody AF2797
  • Human CD5L Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/07/2018
  • Human CD5L Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma and Serum
    Species: Human and Mouse
    Verified Customer | Posted 11/07/2018
  • Human CD5L Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/02/2018

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