CD74 is a transmembrane protein which, on antigen presenting cells, is the invariant chain that chaperones MHC class II dimers from the endoplasmic reticulum to the cell surface. The cytoplasmic portion of CD74 can be released by intramembrane cleavage and activate NF kappa B. CD74 is up-regulated in several cancers and is also expressed by non-immune cells during inflammation. This antibody was raised to a splice variant of human CD74 which lacks the Thyroglobulin type 1 repeat in the extracellular domain. Within comparable regions, human CD74 shares 70% and 73% amino acid sequence identity with mouse and rat CD74, respectively.
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 332516
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Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-S-derived recombinant human CD74
Gln73-Met232
Accession # NP_004346
Gln73-Met232
Accession # NP_004346
Specificity
Detects recombinant human CD74 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human CD74 Antibody
Detection of CD74 in Human PBMC by Flow Cytometry.
Human PBMC lymphocytes were stained with (A) Mouse Anti-Human CD74 Monoclonal Antibody (Catalog # MAB35901) or (B) Mouse IgG1 isotype control (MAB002) followed by anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Human CD19 PE-conjugated Monoclonal Antibody (FAB4867P). Staining was performed using our Staining Membrane-Associated Proteins protocol.
Detection of CD74 in K562 Human Cell Line by Flow Cytometry.
K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human CD74 Monoclonal Antibody (Catalog # MAB35901, filled histogram) or Mouse IgG1 isotype control (MAB002,open histogram) followed by anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-Associated Proteins protocol.
Applications for Human CD74 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human PBMC lymphocytes and K562 myelogenous leukemia cell line
Sample: Human PBMC lymphocytes and K562 myelogenous leukemia cell line
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CD74
Alternate Names
CD74, CLIP, DHLAG, HLADG, Ia-gamma, INVG34
Gene Symbol
CD74
UniProt
Additional CD74 Products
Product Documents for Human CD74 Antibody
Product Specific Notices for Human CD74 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Protocol for the Characterization of Human Th22 Cells
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- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars