Human CD74 Antibody

Catalog # Availability Size / Price Qty
MAB35901
MAB35901-SP

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Detection of CD74 in Human PBMC by Flow Cytometry.
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Human CD74 Antibody Summary

Species Reactivity
Human
Specificity
Detects recombinant human CD74 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 332516
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-S-derived recombinant human CD74
Gln73-Met232
Accession # NP_004346
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD74 antibody in Human PBMC antibody by Flow Cytometry. View Larger

Detection of CD74 in Human PBMC by Flow Cytometry. Human PBMC lymphocytes were stained with (A) Mouse Anti-Human CD74 Monoclonal Antibody (Catalog # MAB35901) or (B) Mouse IgG1 isotype control (MAB002) followed by anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Human CD19 PE-conjugated Monoclonal Antibody (FAB4867P). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Flow Cytometry Detection of CD74 antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CD74 in K562 Human Cell Line by Flow Cytometry. K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human CD74 Monoclonal Antibody (Catalog # MAB35901, filled histogram) or Mouse IgG1 isotype control (MAB002,open histogram) followed by anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD74

CD74 is a transmembrane protein which, on antigen presenting cells, is the invariant chain that chaperones MHC class II dimers from the endoplasmic reticulum to the cell surface. The cytoplasmic portion of CD74 can be released by intramembrane cleavage and activate NF kappa B. CD74 is up-regulated in several cancers and is also expressed by non-immune cells during inflammation. This antibody was raised to a splice variant of human CD74 which lacks the Thyroglobulin type 1 repeat in the extracellular domain. Within comparable regions, human CD74 shares 70% and 73% amino acid sequence identity with mouse and rat CD74, respectively.

Entrez Gene IDs
972 (Human); 16149 (Mouse); 25599 (Rat)
Alternate Names
CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated); CD74 antigen; CD74 molecule, major histocompatibility complex, class II invariant chain; CD74; CLIP; DHLAG; DHLAGgamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLADG; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ia-gamma; Ii; INVG34; MHC HLA-DR gamma chain; p33

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