Human CEACAM-1/CD66a Antibody MAB22441-100: R&D Systems

Human CEACAM-1/CD66a Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CEACAM-1 in ELISAs and Western blots. In ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CD31, rhICAM-1, -2, -3, recombinant mouse MAdCAM-1, or rhVCAM-1 was observed. In sandwich ELISAs, no cross-reactivity with rhCEACAM‑3, rhCEACAM-5, or rhCEACAM-6 was observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 283324
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CEACAM-1
    Gln35-Gly428
    Accession # P13688
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
    • Human CEACAM-1/CD66a Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human CEACAM‑1/CD66a Antibody (Catalog # MAB22441)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human CEACAM‑1/CD66a Biotinylated Antibody (Catalog # BAF2244)
  • ELISA Standard
     
    Recombinant Human CEACAM-1/CD66a Protein, CF (Catalog # 2244-CM)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human CEACAM‑1/CD66a by Western Blot. Western blot shows lysates of human liver tissue and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CEACAM‑1/CD66a Monoclonal Antibody (Catalog # MAB22441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CEACAM‑1/CD66a at approximately 100-150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
CEACAM‑1/CD66a in Human Colon. CEACAM‑1/CD66a was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human CEACAM‑1/CD66a Monoclonal Antibody (Catalog # MAB22441) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
CEACAM‑1/CD66a in Human Colon Cancer Tissue. CEACAM‑1/CD66a was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human CEACAM‑1
/CD66a Monoclonal Antibody (Catalog # MAB22441) at 25 µg/mL over­night at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CEACAM-1/CD66a

Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM-1; also called BGP and designated CD66a) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily (1-3). It is one of seven human CEACAM subfamily genes that are essentially divided equally between type I transmembrane proteins (CEACAM-1, 3, and 4) and GPI-linked molecules (CEACAM-5-8). There is no CEACAM-2 in human. The gene for human CEACAM-1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74 aa cytoplasmic region (4, 5). The ECD contains one N-terminal V-type Ig-like domain, followed by three C2-type Ig-like domains. It shows considerable glycosylation, including high mannose residues and (sialyl) LewisX (1). The cytoplasmic region shows one ITIM motif and a calmodulin binding site (1-3). In addition to the full length form, ten alternate splice forms have been reported (1, 4, 6-8). There are three soluble and seven transmembrane isoforms, with variations occurring in both the ECD and cytoplasmic region. All ten alternate splice forms contain the V‑type Ig-like domain (aa’s 35-142). The three soluble forms also contain the first two C2-type Ig-like domains (aa’s 145-317), with differences coming in the third C2-type Ig-like domain (6). The seven transmembrane isoforms are highly divergent. Five of the seven contain the V-type plus the first two C2-type domains and then diverge considerably both in the ECD and cytoplasmic region. The remaining two contain only the V-type Ig-like domain, the transmembrane region, and either a full-length or truncated cytoplasmic tail (1, 8). The actual functions of the isoforms are unclear. Full-length mouse and rat CEACAM-1 are approximately 57% aa identical to human CEACAM-1; in the V-type Ig-like domain, they are 58% and 56% aa identical, respectively. The full-length molecule is found on neutrophils, bile duct epithelium, activated NK cells, colonic columnar epithelium and endothelium. It is known to act as an intercellular adhesion molecule, forming both homotypic, and heterotypic bonds with CEA and CEACAM-6/NCA (3, 9). On neutrophils, CEACAM-1 also binds to dendritic cell CD-SIGN via its LeX moiety, inducing dendritic cell maturation and a subsequent Th1-type response (10,11).

  • References:
    1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
    2. Thompson, J. et al. (1992) Genomics 12:761.
    3. Waggener, C. and S. Ergun (2000) Exp. Cell Res. 261:19.
    4. Barnett, T.R. et al. (1989) J. Cell Biol. 108:267.
    5. Hinoda, Y. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6959.
    6. Kuroki, M. et al. (1991) Biochem. Biophys. Res. Commun. 176:578.
    7. Barnett, T.R. et al. (1993) Mol. Cell. Biol. 13:1273.
    8. Watt, S.M. et al. (1994) Blood 84:200.
    9. Oikawa, S. et al. (1992) Biochem. Biophys. Res. Commun. 186:881.
    10. Klaas, P.J.M. et al. (2005) FEBS Lett. 579:6159.
    11. Bogoevska, V. et al. (2005) Glycobiology 16:197.
  • Long Name:
    Carcinoembryonic Antigen-related Cell Adhesion Molecule 1
  • Entrez Gene IDs:
    634 (Human); 26365 (Mouse)
  • Alternate Names:
    antigen CD66; BGP1; BGP-1; BGP1BGPBiliary glycoprotein 1; BGPI; Biliary Glycoprotein 1; biliary glycoprotein adhesion molecule; carcinoembryonic antigen-related cell adhesion molecule 1 (biliaryglycoprotein); carcinoembryonic antigen-related cell adhesion molecule 1; CD66a antigen; CD66a; Cea-1; CEACAM1; CEACAM-1; Hv-1; Hv-2; MHVR
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Serum CEACAM1 Level Is Associated with Diagnosis and Prognosis in Patients with Osteosarcoma
    Authors: H Yu, J Yu, Y Ren, Y Yang, X Xiao
    PLoS ONE, 2016;11(4):e0153601.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  2. AP-1-controlled hepatocyte growth factor activation promotes keratinocyte migration via CEACAM1 and urokinase plasminogen activator/urokinase plasminogen receptor.
    Authors: Schnickmann S, Camacho-Trullio D, Bissinger M, Eils R, Angel P, Schirmacher P, Szabowski A, Breuhahn K
    J. Invest. Dermatol., 2009;129(5):1140-8.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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Isotype Controls
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Mouse IgG1 Isotype Control

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Mouse IgG1 Isotype Control

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Mouse IgG HRP-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

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Donkey Anti-Mouse IgG Biotinylated Antibody

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Mouse IgG HRP-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

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Mouse IgG Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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