Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Choline Acetyltransferase/ChAT Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Choline Acetyltransferase/ChAT isoform R
Ala2-Pro630
Accession # NP_066266

Specificity

Detects human Choline Acetyltransferase/ChAT in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Choline Acetyltransferase/ChAT Antibody

Choline Acetyltransferase/ChAT antibody in Human Brain by Immunohistochemistry (IHC-P).

Choline Acetyltransferase/ChAT in Human Brain.

Choline Acetyltransferase/ChAT was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 15 µg/mL Goat Anti-Human Choline Acetyltransferase/ChAT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3447) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Choline Acetyltransferase/ChAT Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Western Blot

0.1 µg/mL
Sample: Recombinant Human Choline Acetyltransferase/ChAT Isoform R

Reviewed Applications

Read 2 reviews rated 4.5 using AF3447 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Choline Acetyltransferase/ChAT

ChAT catalyzes the production of the neurotransmitter acetylcholine (Ach), which is required for cholinergic neuron communication. It serves as a marker for functional cholinergic neurons in the central and peripheral nervous systems. At least six ChAT mRNAs encoding 69 kDa, 82 kDa and 74 kDa ChAT proteins have been identified. Compared to the 82 kDa form, 69 kDa ChAT lacks the N-terminal 118 amino acid extension containing a nuclear localization signal. As a result the 69 kDa ChAT is primarily localized to the cytoplasm. Human 69 kDa ChAT shares 86% amino acid sequence identity with the mouse or rat ChAT.

Alternate Names

CHAT, Choline acetylase

Entrez Gene IDs

1103 (Human); 12647 (Mouse); 290567 (Rat)

Gene Symbol

CHAT

UniProt

NP_066266

Additional Choline Acetyltransferase/ChAT Products

Product Documents for Human Choline Acetyltransferase/ChAT Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Choline Acetyltransferase/ChAT Antibody

For research use only

Citations for Human Choline Acetyltransferase/ChAT Antibody

Customer Reviews for Human Choline Acetyltransferase/ChAT Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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3 Stars
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1 Stars
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  • Human Choline Acetyltransferase/ChAT Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: mouse paraformadehyde fixed brain sections and mouse 4% paraformaldehyde fixed brain sections
    Species: Mouse
    Verified Customer | Posted 10/20/2016
    1:4000 dilution on brain sections pretreated with 1% NaBH4.
    Human Choline Acetyltransferase/ChAT Antibody AF3447
  • Human Choline Acetyltransferase/ChAT Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Liver tissue, Brain tissue and Small intestine
    Species: Human and Mouse
    Verified Customer | Posted 06/01/2016
    Primary ab dilution 1:4000 (better with 1:2000) 40 ug protein per lane
    Human Choline Acetyltransferase/ChAT Antibody AF3447

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Protocols

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