Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 396305
View all Chondrolectin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Chondrolectin
Arg22-Asn216
Accession # Q9H9P2

Specificity

Detects human Chondrolectin in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Chondrolectin Antibody

Detection of Chondrolectin antibody in PC‑3 Human Cell Line antibody by Flow Cytometry.

Detection of Chondrolectin in PC‑3 Human Cell Line by Flow Cytometry.

PC-3 human prostate cancer cell line was stained with Mouse Anti-Human Chondrolectin Monoclonal Antibody (Catalog # MAB2576, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).
Chondrolectin antibody in PC-3 Human Cell Line by Immunocytochemistry (ICC).

Chondrolectin in PC‑3 Human Cell Line.

Chondrolectin was detected in immersion fixed PC-3 human prostate cancer cell line using Mouse Anti-Human Chondrolectin Monoclonal Antibody (Catalog # MAB2576) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Chondrolectin Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: PC‑3 human prostate cancer cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed PC-3 human prostate cancer cell line

Western Blot

1 µg/mL
Sample: Recombinant Human Chondrolectin

Reviewed Applications

Read 1 review rated 5 using MAB2576 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chondrolectin

Chondrolectin (CHODL) is a 36 kDa type I transmembrane protein containing a C-type lectin carbohydrate recognition domain. Multiple isoforms of human CHODL are generated as a result of alternative promoter usage and/or alternative splicing. Some isoforms are differentially expressed during T cell development. Human and mouse CHODL share 94% amino acid sequence identity in their extracellular region.

Alternate Names

C21orf68, CHODL, MT75, PRED12

Entrez Gene IDs

140578 (Human); 246048 (Mouse)

Gene Symbol

CHODL

UniProt

Q9H9P2

Additional Chondrolectin Products

Product Documents for Human Chondrolectin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Chondrolectin Antibody

For research use only

Related Research Areas

C-type Lectin Family

Customer Reviews for Human Chondrolectin Antibody (1)

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  • Human Chondrolectin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: PC-3 human prostate cancer cell line
    Species: Human
    Verified Customer | Posted 04/11/2022
    Human Chondrolectin Antibody MAB2576

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Protocols

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