Detection of Chondrolectin in PC‑3 Human Cell Line by Flow Cytometry.
PC‑3 human prostate cancer cell line was stained with Mouse Anti-Human Chondrolectin Monoclonal Antibody (Catalog # MAB2576, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).
Chondrolectin in PC‑3 Human Cell Line.
Chondrolectin was detected in immersion fixed PC‑3 human prostate cancer cell line using Mouse Anti-Human Chondrolectin Monoclonal Antibody (Catalog # MAB2576) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Chondrolectin (CHODL) is a 36 kDa type I transmembrane protein containing a C-type lectin carbohydrate recognition domain. Multiple isoforms of human CHODL are generated as a result of alternative promoter usage and/or alternative splicing. Some isoforms are differentially expressed during T cell development. Human and mouse CHODL share 94% amino acid sequence identity in their extracellular region.
Entrez Gene IDs:
C21orf68; CHODL; Chondrolectin; chromosome 21 open reading frame 68; FLJ12627; MT75; PRED12; Transmembrane protein MT75
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