cIAP‑1/HIAP‑2 in Human Prostate. cIAP‑1/HIAP‑2 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human cIAP‑1/HIAP‑2 Monoclonal Antibody (Catalog # MAB8181) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
(also known as BIR2, MIHB and HIAP-2) is a member of the inhibitor of
apoptosis (IAP) family of proteins that inhibit the proteolytic activity
of mature caspases. cIAP-1 has 3 BIR (baculovirus inhibitor of
apoptosis) domains, a RING finger domain, and a caspase recruitment
domain (CARD). cIAP-1 inhibits caspases by interaction of the BIR domain
with the active caspase. Caspase activity may be restored through
interactions with the Reaper like motif on mitochondrial proteins such
as SMAC/Diablo or HTRA-2/Omi. cIAP-1 is reported to be cleaved by
caspases in fetal rat hepatocytes treated with TGF-beta.
Roy, N. et al. (1997) EMBO J. 23:6914.
Deveraux, Q. et al. (1997) Nature 388:300.
Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
Herrera, B. et al. (2002) FEBS Letters 520:93.
Cellular Inhibitor of Apoptosis Protein 1
Entrez Gene IDs:
329 (Human); 11797 (Mouse)
API1Hiap-2; apoptosis inhibitor 1; baculoviral IAP repeat containing 2; baculoviral IAP repeat-containing 2; baculoviral IAP repeat-containing protein 2; BIRC2; cIAP1; c-IAP1; hIAP2; HIAP-2; IAP homolog B; IAP2; IAP-2; Inhibitor of apoptosis protein 2; MIHB; MIHBHIAP2; NFR2-TRAF signalling complex protein; RING finger protein 48; RNF48hiap-2; TNFR2-TRAF-signaling complex protein 2
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