Human cIAP-1/HIAP-2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human cIAP-1/HIAP-2 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) cIAP-2 (aa 2-604) and no cross-reactivity with rhBIRC6 (aa 4582-4735), rhcIAP-2 (aa 94-178), rhXIAP (aa 1-497), rhXIAP (BIR2 domain; aa 124-242), or rhXIAP (BIR3 domain; aa 252-356) is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 681732
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human cIAP-1/HIAP-2
    His2-Ser618
    Accession # Q13490
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human cIAP‑1/HIAP‑2 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected, transfected with full length human cIAP-1, or transfected with full length human cIAP-2, HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human cIAP‑1/HIAP‑2 Monoclonal Antibody (Catalog # MAB818) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for cIAP‑1/HIAP‑2 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: cIAP-1/HIAP-2

cIAP-1 (also known as BIR2, MIHB and HIAP-2) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-1 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-1 inhibits caspases by interaction of the BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HTRA-2/Omi. cIAP-1 is reported to be cleaved by caspases in fetal rat hepatocytes treated with TGF-beta.

  • References:
    1. Roy, N. et al. (1997) EMBO J. 23:6914.
    2. Deveraux, Q. et al. (1997) Nature 388:300.
    3. Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
    4. Herrera, B. et al. (2002) FEBS Letters 520:93.
  • Long Name:
    Cellular Inhibitor of Apoptosis Protein 1
  • Entrez Gene IDs:
    329 (Human); 11797 (Mouse)
  • Alternate Names:
    API1Hiap-2; apoptosis inhibitor 1; baculoviral IAP repeat containing 2; baculoviral IAP repeat-containing 2; baculoviral IAP repeat-containing protein 2; BIRC2; cIAP1; c-IAP1; cIAP-1; hIAP2; HIAP-2; IAP homolog B; IAP2; IAP-2; Inhibitor of apoptosis protein 2; MIHB; MIHBHIAP2; NFR2-TRAF signalling complex protein; RING finger protein 48; RNF48hiap-2; TNFR2-TRAF-signaling complex protein 2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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