Human Claudin-11 Antibody

Catalog #: MAB11716 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11716-100
MAB11716-SP

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Detection of Claudin‑11 in Human Testis.

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All Claudin-11 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Claudin-11 Antibody Summary

Species Reactivity

Human

Specificity

Detects a synthetic peptide specific for human Claudin-11 around amino acid 200 in Direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1102132

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic Peptide
Accession # O75508

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

3-25 µg/mL

Immersion fixed paraffin-embedded sections of human testis

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of Claudin‑11 in Human Testis. Claudin‑11 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human Claudin‑11 Monoclonal Antibody (Catalog # MAB11716) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Claudin-11

Claudin-11 is a 207 amino acid multipass membrane protein in the claudin family of epithelial tight junction proteins. Claudin-11 is widely expressed and is involved in epithelial remodeling during spermatogenesis and inner ear mechanotransduction. Claudin-11 is up‑regulated in some cancers. Human Claudin-11 shares 94% aa sequence identity with mouse and rat Claudin-11.

Entrez Gene IDs

5010 (Human); 18417 (Mouse); 84588 (Rat)

Alternate Names

claudin 11; Claudin11; Claudin-11; CLDN11; Oligodendrocyte-specific protein; OSP; OSPOTMoligodendrocyte transmembrane protein; OTM