Human CLEC14A Antibody

Catalog # Availability Size / Price Qty
MAB7436
MAB7436-SP
Detection of CLEC14A in HUVEC Human Cells by Flow Cytometry.
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Human CLEC14A Antibody Summary

Species Reactivity
Human
Specificity
Detects human CLEC14A in direct ELISA.
Source
Monoclonal Mouse IgG2B Clone # 743940
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CLEC14A
Glu22-Ala397
Accession # Q86T13
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CLEC14A antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of CLEC14A in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human CLEC14A Monoclonal Antibody (Catalog # MAB7436, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Immunocytochemistry CLEC14A antibody in HUVEC Human Cells by Immunocytochemistry (ICC). View Larger

CLEC14A in HUVEC Human Cells. CLEC14A was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human CLEC14A Monoclonal Antibody (Catalog # MAB7436) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC14A

CLEC14A (C-type Lectin domain family 14 member A), also known as EGFR5, is a 51 kDa (predicted) member of the C-type lectin domain family of proteins. It is a type I transmembrane protein, apparently expressed in brain. Mature human CLEC14A is 469 amino acids (aa) in length. It contains a 376 aa extracellular region (aa 22-397) and a 72 aa cytoplasmic domain. The extracellular region shows one C-type lectin like domain (aa 32-175) and an EGF-like region (aa 245-287). Over aa 22-397, human CLEC14A shares 66% and 81% aa identity with mouse and canine CLEC14A, respectively.

Long Name
C-type Lectin Domain Family 14, Member A
Entrez Gene IDs
161198 (Human); 66864 (Mouse); 314148 (Rat)
Alternate Names
C14orf27; CEG1; chromosome 14 open reading frame 27; CLEC14A; ClECT and EGF-like domain containing protein; C-type lectin domain family 14 member A; C-type lectin domain family 14, member A; EGF R5; EGFR5; EGFR-5; Epidermal growth factor receptor 5

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human CLEC14A Antibody

There are currently no reviews for this product. Be the first to review Human CLEC14A Antibody and earn rewards!

Have you used Human CLEC14A Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review