Human Collagen XIII  alpha 1 Antibody

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Collagen XIII  alpha 1 in Human Placenta.
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Human Collagen XIII  alpha 1 Antibody Summary

Species Reactivity
Detects human Collagen XIII alpha 1 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse Collagen XIII alpha 1 is observed.
Monoclonal Mouse IgG2B Clone # 584718
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human Collagen XIII alpha 1
Accession # Q5TAT6
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Collagen XIII a1 antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

Collagen XIII alpha 1 in Human Placenta. Collagen XIII a1 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Collagen XIII a1 Monoclonal Antibody (Catalog # MAB6346) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to basement membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collagen XIII alpha 1

COL13A1 (collagen 13-alpha 1) is a 95 kDa member of the transmembrane group of the collagen family of proteins. It is a type II transmembrane glycoprotein that is expressed by multiple cell types, including fibroblasts, endothelial cells, and cardiac muscle. COL13A1 forms disulfide-linked homotrimers and participates in cell adhesion by binding to the Integrin alpha 1 subunit, Nidogen-2, and Fibronectin. Human COL13A1 is 717 amino acids (aa) in length. It has an N‑terminal 44 aa cytoplasmic region plus a 656 aa extracellular domain (ECD) (aa 62-717). The ECD contains four non-collagenous (NC) regions (aa 1‑121, aa 217-269, aa 442-463, and aa 700-717) interspersed among three collagenous (COL) domains (aa 122-216, aa 270-441, and aa 464-699). Multiple splice forms exist and typically involve deletions of 12-30 aa between aa 220-705. Proteolytic cleavage after Arg108 generates an 85-90 kDa soluble form. Over aa 109-668 (based on a human isoform [NP_542992} that shows a deletion of aa 239-260, aa 551-565, and aa 616-627), human COL13A1 shares 91% aa sequence identity with mouse COL13A1.

Entrez Gene IDs
1305 (Human); 12817 (Mouse); 499431 (Rat)
Alternate Names
COL13A1; collagen alpha-1(XIII) chain; Collagen XIII alpha 1; collagen, type XIII, alpha 1; FLJ42485

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