Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CREB Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CREB
Lys136-Asp341
Accession # P16220

Specificity

Detects human CREB in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CREB Antibody

Detection of Human CREB antibody by Western Blot.

Detection of Human CREB by Western Blot.

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, HepG2 human hepatocellular carcinoma cell line, MBA-MB-468 human breast cancer cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human CREB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2989) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CREB at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of CREB-regulated Genes antibody by Chromatin Immunoprecipitation.

Detection of CREB-regulated Genes by Chromatin Immunoprecipitation.

Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. CREB/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human CREB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2989) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thefospromoter was detected by standard PCR.

CREB antibody in Human Breast by Immunohistochemistry (IHC-P).

CREB in Human Breast.

CREB was detected in immersion fixed paraffin-embedded sections of human breast using 10 µg/mL Goat Anti-Human CREB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2989) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the nucleus in stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

CREB antibody in Human Dorsal Root Ganglion by Immunohistochemistry (IHC-P).

CREB in Human Dorsal Root Ganglion.

CREB was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglion using Goat Anti-Human CREB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2989) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

CREB antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

CREB in MCF‑7 Human Cell Line.

CREB was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human CREB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2989) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

CREB antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

CREB in MCF‑7 Human Cell Line.

CREB was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human CREB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2989) at 0.5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human CREB Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

5 µg/5 x 106 cells
Sample: PMA and calcium ionomycin treated Jurkat human acute T cell leukemia cell line chromatin, fos promoter detected by standard PCR.

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast and dorsal root ganglion

Western Blot

0.5 µg/mL
Sample: HT-29 human colon adenocarcinoma cell line, HepG2 human hepatocellular carcinoma cell line, MBA-MB-468 human breast cancer cell line, and MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CREB

The cAMP response element binding protein (CREB), also known as CREB1, belongs to the bZIP superfamily of transcription factors, containing a basic domain that mediates DNA binding and a leucine zipper domain that facilitates dimerization. Within the promoter of target genes, CREB dimers bind cAMP response elements, defined by the palindromic consensus sequence TGACGTCA.

Long Name

cAMP Response Element-binding Protein

Alternate Names

CREB1

Entrez Gene IDs

1385 (Human); 12912 (Mouse)

Gene Symbol

CREB1

UniProt

P16220

Additional CREB Products

Product Documents for Human CREB Antibody

Certificate of Analysis

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Product Specific Notices for Human CREB Antibody

For research use only

Citations for Human CREB Antibody

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Protocols

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