Human CXCL11/I-TAC Antibody

R&D Systems | Catalog # AF260

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunohistochemistry-Frozen, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CXCL11/I-TAC Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CXCL11/I-TAC
Phe22-Phe94
Accession # O14625

Specificity

Detects human CXCL11/I-TAC in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouseI-TAC is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CXCL11/I-TAC Antibody

Chemotaxis Induced by CXCL11/I‑TAC and Neutralization by Human CXCL11/I‑TAC Antibody.

Chemotaxis Induced by CXCL11/I‑TAC and Neutralization by Human CXCL11/I‑TAC Antibody.

Recombinant Human CXCL11/I-TAC (Catalog # 672-IT) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL11/I-TAC (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL11/I-TAC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF260). The ND50 is typically 0.5-1.5 µg/mL.
Detection of Human CXCL11/I-TAC by Block/Neutralize

Detection of Human CXCL11/I-TAC by Block/Neutralize

CXCR7-mediated Rb degradation requires ligand.pLEC cultures were infected with Trans only or Trans+CXCR7. At 6 hours post-infection, media was replaced with media only or media containing (A) SDF-1/CXCL12 neutralizing antibody at 1.5, or 3.0 µg/ml or (B) ITAC/CXCL11 neutralizing antibody at 0.5 or 1.0 µg/ml. At 20 hours post infection, cells were lysed and analyzed by western blot for total Rb levels. Densitometry analysis was performed and fold change values were calculated from GAPDH-normalized optical density ratios as described for Figure 3. Bar graphs (bottom) represent the average fold change at each condition for two independent experiments. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0069828), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human CXCL11/I-TAC by Block/Neutralize

Detection of Human CXCL11/I-TAC by Block/Neutralize

CXCR7-mediated Rb degradation requires ligand.pLEC cultures were infected with Trans only or Trans+CXCR7. At 6 hours post-infection, media was replaced with media only or media containing (A) SDF-1/CXCL12 neutralizing antibody at 1.5, or 3.0 µg/ml or (B) ITAC/CXCL11 neutralizing antibody at 0.5 or 1.0 µg/ml. At 20 hours post infection, cells were lysed and analyzed by western blot for total Rb levels. Densitometry analysis was performed and fold change values were calculated from GAPDH-normalized optical density ratios as described for Figure 3. Bar graphs (bottom) represent the average fold change at each condition for two independent experiments. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0069828), licensed under a CC-BY license. Not internally tested by R&D Systems.
Human CXCL11 / I-TAC ELISA Standard Curve

Human CXCL11 / I-TAC ELISA Standard Curve

Recombinant Human CXCL11/I‑TAC (Catalog # 672-IT) was serially diluted and captured by Mouse Anti-Human CXCL11/I‑TAC Monoclonal Antibody (Catalog # MAB672) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL11/I‑TAC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF260) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CXCL11/I-TAC Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human CXCL11/I-TAC (Catalog # 672-IT)

Neutralization

Measured by its ability to neutralize CXCL11/I‑TAC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 0.5-1.5 µg/mL in the presence of 0.05 µg/mL Recombinant Human CXCL11/I‑TAC.

Reviewed Applications

Read 1 review rated 5 using AF260 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL11/I-TAC

CXCL11, also known as I-TAC, SCYB9B, H174 and beta -R1, is a non-ELR CXC chemokine. CXCL11 cDNA encodes a 94 amino acid (aa) residue precursor protein with a 21 aa residue putative signal sequence, which is cleaved to form the mature 73 aa residue protein. CXCL11 shares 36% and 37% amino acid sequence homology with IP-10 and MIG (two other known human non-ELR CXC chemokines), respectively. CXCL11 is expressed at low levels in normal tissues including thymus, spleen and pancreas. The expression of CXCL11 mRNA is radically up regulated in IFN-gamma and IL-1 stimulated astrocytes. Moderate increase in expression is also observed in stimulated monocytes. CXCL11 has potent chemoattractant activity for IL-2 activated T cells and transfected cell lines expressing CXCR3, but not freshly isolated T cells, neutrophils, or monocytes. The gene encoding CXCL11 has been mapped to chromosome 4.

References

  1. Cole, K. et al. (1998) J. Exp. Med. 187:2009.
  2. Sandhya Rani, M. et al. (1996) J. Biol. Chem. 271:22878.
  3. Lou, Y. et al. (1998) J. Neurovirol. 4:575.

Alternate Names

beta-R1, H174, I-TAC, ITAC, SCYB9B

Entrez Gene IDs

6373 (Human); 56066 (Mouse)

Gene Symbol

CXCL11

UniProt

O14625

Additional CXCL11/I-TAC Products

Product Documents for Human CXCL11/I-TAC Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CXCL11/I-TAC Antibody

For research use only

Citations for Human CXCL11/I-TAC Antibody

Customer Reviews for Human CXCL11/I-TAC Antibody (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 19552626
    Species: Human
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