Human CXCL12/SDF-1 Antibody

R&D Systems | Catalog # AF-310-NA

R&D Systems
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Zebrafish

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Immunocytochemistry, Bioassay, Blocking/Neutralizing, ELISA Development (Capture), Functional Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CXCL12/SDF-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CXCL12/SDF‑1 alpha
Lys22-Lys89
Accession # Q6ICW0

Specificity

Detects human CXCL12/SDF-1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CXCL12/SDF-1 Antibody

Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutralization by Human CXCL12/SDF‑1 Antibody.

Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutralization by Human CXCL12/SDF‑1 Antibody.

Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (Catalog # 350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL12/SDF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA). The ND50 is typically 1.5-6.0 µg/mL.
Human CXCL12 / SDF-1 ELISA Standard Curve

Human CXCL12 / SDF-1 ELISA Standard Curve

Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (Catalog # 350-NS) was serially diluted and captured by Mouse Anti-Human/Mouse CXCL12/SDF‑1 Monoclonal Antibody (Catalog # MAB350) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL12/SDF‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Mouse CXCL12 / SDF-1 ELISA Standard Curve

Mouse CXCL12 / SDF-1 ELISA Standard Curve

Recombinant Mouse CXCL12/SDF‑1 alpha (Catalog # 460-SD) was serially diluted and captured by Mouse Anti-Human/Mouse CXCL12/SDF‑1 Monoclonal Antibody (Catalog # MAB350) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL12/SDF‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CXCL12/SDF-1 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human/Feline CXCL12/SDF-1 beta aa 19-93 (Catalog # 2716-SD)
Recombinant Human/Feline CXCL12/SDF-1 beta aa 22-93 (Catalog # 351-FS)
Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1 alpha (Catalog # 350-NS)

Neutralization

Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. The Neutralization Dose (ND50) is typically 1.5-6.0 µg/mL in the presence of 2 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha.

Reviewed Applications

Read 2 reviews rated 4 using AF-310-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CXCL12/SDF-1

CXCL12, also known as SDF-1 (stromal cell-derived factor 1) and PBSF (Pre-B cell growth stimulatory factor), is a member of the CXC chemokine superfamily. Two alternatively spliced mRNAs encode CXCL12 alpha (89 aa residues) and CXCL12 beta (93 aa residues) that differ by the addition of 4 amino acids at the carboxy-terminus. Human and mouse CXCL12 are highly conserved. Mature human and mouse CXCL12 differ only by 1 amino acid.

Alternate Names

PBSF, SDF-1, SDF1

Entrez Gene IDs

6387 (Human); 20315 (Mouse); 493806 (Feline); 574334 (Rhesus Macaque)

Gene Symbol

CXCL12

UniProt

Q6ICW0

Additional CXCL12/SDF-1 Products

Product Documents for Human CXCL12/SDF-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CXCL12/SDF-1 Antibody

For research use only

Citations for Human CXCL12/SDF-1 Antibody

Customer Reviews for Human CXCL12/SDF-1 Antibody (2)

4 out of 5
2 Customer Ratings
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human CXCL12/SDF-1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: See PMID 23009808
    Species: Human
    Verified Customer | Posted 01/07/2015
  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: See PMID 23009808
    Species: Other
    Verified Customer | Posted 01/07/2015

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies