hh Human CXCL7/NAP-2 Antibody MAB393: R&D Systems

Human CXCL7/NAP-2 Antibody

(3 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CXCL7/NAP‑2 in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human (rh) CXCL1, 2, 3, 5, 6, 8, 9, 10, or 12 is observed. In Western blots, no cross-reactivity with rhCXCL1, 2, 3, 5, 6, 8, 9, 10, 12, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12 or recombinant rat CXCL1, 2 or 3 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 59418
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human CXCL7/NAP-2
    Ala59-Asp128
    Accession # P02775
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human CXCL7/NAP‑2 (Catalog # 393-NP)
  • Simple Western
    10 µg/mL
    See below
    • Human CXCL7/NAP-2 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human CXCL7/NAP‑2 Antibody (Catalog # MAB393)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human CXCL7/NAP‑2 Biotinylated Antibody (Catalog # BAF393)
  • ELISA Standard
     
    Recombinant Human CXCL7/NAP-2 Protein (Catalog # 393-NP)
  • Neutralization
    Measured by its ability to neutralize CXCL7/NAP‑2-induced myeloperoxidase release in cytochalasin‑B treated human neutrophils. Schröder, J. M. et al. (1987) J.  Immunol. 139:3474. The Neutralization Dose (ND50) is typically 15-20 µg/mL in the presence of 1 µg/mL Recombinant Human CXCL7/NAP‑2 and cytochalasin‑B.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Myeloperoxidase Release Induced by CXCL7/NAP‑2 and Neutralization by Human CXCL7/NAP‑2 Antibody. Recombinant Human CXCL7/NAP‑2 (Catalog #
393‑NP) induces myeloperox­idase release in the cytochalasin‑B treated human neutrophils in a dose-dependent manner (orange line). Myeloperoxidase Release elicited by Recombinant Human CXCL7/NAP‑2 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL7/
NAP‑2 Monoclonal Antibody (Catalog # MAB393). The ND50 is typically 15‑20 µg/mL in the presence of cytochalasin‑B.
Detection of Human CXCL7/NAP‑2 by Simple WesternTM. Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for CXCL7/NAP‑2 at approximately 10 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human CXCL7/NAP‑2 Monoclonal Antibody (Catalog # MAB393). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL7/NAP-2
CXCL7, also known as NAP-2, is a 11 kDa angiogenic alpha -chemokine that is produced by the proteolytic cleavage of the inactive 13 kDa platelet basic protein (PBP). PBP, CXCL7, and C-terminally truncated forms of CXCL7 are secreted by monocytes and platelets. Active CXCL7 binds CXCR2 and induces the chemoattration of monocytes, neutrophils, and endothelial progenitor cells. It also inhibits megakaryocytopoiesis in the bone marrow. Within the region included in this immunogen, human CXCL7 shares 52% aa sequence identity with mouse and rat CXCL7.
  • Entrez Gene IDs:
    5473 (Human)
  • Alternate Names:
    Beta-TG; connective tissue-activating peptide III; CTAP III; CTAP3CTAP-III; CTAPIII; CXC chemokine ligand 7; C-X-C motif chemokine 7; CXCL7; CXCL7b-TG1; LA-PF4; LDGFTC1; Leukocyte-derived growth factor; low-affinity platelet factor IV; Macrophage-derived growth factor; MDGFTC2; NAP2; NAP-2; NAP-2-L1; neutrophil-activating peptide 2; neutrophil-activating peptide-2; PBPTHBGB; platelet basic protein; PPBP; pro-platelet basic protein (chemokine (C-X-C motif) ligand 7); SCAR10; SCYB7beta-thromboglobulin; small inducible cytokine B7; small inducible cytokine subfamily B, member 7; Small-inducible cytokine B7; TGB; TGB1B-TG1; THBGB1; thrombocidin 1; thrombocidin 2; thromboglobulin, beta-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Up-regulation of platelet activation in hemophilia A.
    Authors: van Bladel ER, Roest M
    Haematologica, 2011;0(0):.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  2. High platelet reactivity is associated with myocardial infarction in premenopausal women: a population-based case-control study.
    Authors: Snoep JD, Roest M, Barendrecht AD
    J. Thromb. Haemost., 2010;8(5):906-13.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  3. Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation.
    Authors: Smith C, Damas JK, Otterdal K, ØIe E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Froland SS, Hansson GK, Aukrust P
    J. Am. Coll. Cardiol., 2006;48(8):1591-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Block/Neutralize.

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 Block/Neutralize Human Anonymous 04/26/2017
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Summary

ApplicationBlock/Neutralize
Sample TestedPeripheral blood neutrophils
SpeciesHuman

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