Human CXCL7/NAP-2 Antibody

Catalog # Availability Size / Price Qty
Myeloperoxidase Release Induced by CXCL7/NAP‑2 and Neutralization by Human CXCL7/NAP‑2 Antibody.
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Product Details
Citations (5)
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Human CXCL7/NAP-2 Antibody Summary

Species Reactivity
Detects human CXCL7/NAP‑2 in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human (rh) CXCL1, 2, 3, 5, 6, 8, 9, 10, or 12 is observed. In Western blots, no cross-reactivity with rhCXCL1, 2, 3, 5, 6, 8, 9, 10, 12, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12 or recombinant rat CXCL1, 2 or 3 is observed.
Monoclonal Mouse IgG1 Clone # 59418
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human CXCL7/NAP-2
Accession # P02775
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human CXCL7/NAP‑2 (Catalog # 393-NP)
Simple Western
10 µg/mL
See below

Human CXCL7/NAP-2 Sandwich Immunoassay

Recommended Concentration
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human CXCL7/NAP‑2 Biotinylated Antibody (Catalog # BAF393)

Standard: Recombinant Human CXCL7/NAP-2 Protein (Catalog # 393-NP)

Measured by its ability to neutralize CXCL7/NAP‑2-induced myeloperoxidase release in cytochalasin‑B treated human neutrophils. Schröder, J. M. et al. (1987) J.  Immunol. 139:3474. The Neutralization Dose (ND50) is typically 15-20 µg/mL in the presence of 1 µg/mL Recombinant Human CXCL7/NAP‑2 and cytochalasin‑B.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Myeloperoxidase Release Induced by CXCL7/NAP‑2 and Neutralization by Human CXCL7/NAP‑2 Antibody. View Larger

Myeloperoxidase Release Induced by CXCL7/NAP‑2 and Neutralization by Human CXCL7/NAP‑2 Antibody. Recombinant Human CXCL7/NAP-2 (Catalog # 393-NP) induces myeloperox-idase release in the cytochalasin-B treated human neutrophils in a dose-dependent manner (orange line). Myeloperoxidase Release elicited by Recombinant Human CXCL7/NAP-2 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL7/ NAP-2 Monoclonal Antibody (Catalog # MAB393). The ND50 is typically 15-20 µg/mL in the presence of cytochalasin-B.

Simple Western Detection of Human CXCL7/NAP‑2 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human CXCL7/NAP‑2 by Simple WesternTM. Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for CXCL7/NAP‑2 at approximately 10 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human CXCL7/NAP‑2 Monoclonal Antibody (Catalog # MAB393). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL7/NAP-2

CXCL7, also known as NAP-2, is a 11 kDa angiogenic alpha -chemokine that is produced by the proteolytic cleavage of the inactive 13 kDa platelet basic protein (PBP). PBP, CXCL7, and C-terminally truncated forms of CXCL7 are secreted by monocytes and platelets. Active CXCL7 binds CXCR2 and induces the chemoattration of monocytes, neutrophils, and endothelial progenitor cells. It also inhibits megakaryocytopoiesis in the bone marrow. Within the region included in this immunogen, human CXCL7 shares 52% aa sequence identity with mouse and rat CXCL7.

Entrez Gene IDs
5473 (Human)
Alternate Names
Beta-TG; connective tissue-activating peptide III; CTAP III; CTAP3CTAP-III; CTAPIII; CXC chemokine ligand 7; C-X-C motif chemokine 7; CXCL7; CXCL7b-TG1; LA-PF4; LDGFTC1; Leukocyte-derived growth factor; low-affinity platelet factor IV; Macrophage-derived growth factor; MDGFTC2; NAP2; NAP-2; NAP-2-L1; neutrophil-activating peptide 2; neutrophil-activating peptide-2; PBPTHBGB; platelet basic protein; PPBP; pro-platelet basic protein (chemokine (C-X-C motif) ligand 7); SCAR10; SCYB7beta-thromboglobulin; small inducible cytokine B7; small inducible cytokine subfamily B, member 7; Small-inducible cytokine B7; TGB; TGB1B-TG1; THBGB1; thrombocidin 1; thrombocidin 2; thromboglobulin, beta-1

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Citations for Human CXCL7/NAP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Development of a Glycosaminoglycan Derived, Selectin Targeting Anti-Adhesive Coating to Treat Endothelial Cell Dysfunction
    Authors: JR Wodicka, AM Chambers, GS Sangha, CJ Goergen, A Panitch
    Pharmaceuticals (Basel), 2017;10(2):.
    Species: Human
    Sample Types: Platelet Rich Plasma (PRP)
    Applications: ELISA Development (Capture)
  2. Effect of platelet-derived ?-thromboglobulins on coagulation
    Authors: K Egan, JP van Geffen, H Ma, B Kevane, A Lennon, S Allen, E Neary, M Parsons, P Maguire, K Wynne, R O' Kennedy, JW Heemskerk, FN Áinle
    Thromb. Res., 2017;154(0):7-15.
    Species: Human
    Sample Types: Plasma
    Applications: Functional Assay
  3. Up-regulation of platelet activation in hemophilia A.
    Authors: van Bladel ER, Roest M
    Haematologica, 2011;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  4. High platelet reactivity is associated with myocardial infarction in premenopausal women: a population-based case-control study.
    Authors: Snoep JD, Roest M, Barendrecht AD
    J. Thromb. Haemost., 2010;8(5):906-13.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  5. Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation.
    Authors: Smith C, Damas JK, Otterdal K, ØIe E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Froland SS, Hansson GK, Aukrust P
    J. Am. Coll. Cardiol., 2006;48(8):1591-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization


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Human CXCL7/NAP-2 Antibody
By Michelle Chen on 04/26/2017
Application: B/N Sample Tested: Peripheral blood neutrophils Species: Human