Detects human CXCL7/NAP‑2 in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human (rh) CXCL1, 2, 3, 5, 6, 8, 9, 10, or 12 is observed. In Western blots, no cross-reactivity with rhCXCL1, 2, 3, 5, 6, 8, 9, 10, 12, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12 or recombinant rat CXCL1, 2 or 3 is observed.
Monoclonal Mouse IgG1 Clone # 59418
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human CXCL7/NAP-2 Ala59-Asp128 Accession # P02775
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Human CXCL7/NAP‑2 Biotinylated Antibody (Catalog # BAF393)
Recombinant Human CXCL7/NAP-2 Protein (Catalog # 393-NP)
Measured by its ability to neutralize CXCL7/NAP‑2-induced myeloperoxidase release in cytochalasin‑B treated human neutrophils. Schröder, J. M. et al. (1987) J. Immunol. 139:3474. The Neutralization Dose (ND50) is typically 15-20 µg/mL in the presence of 1 µg/mL Recombinant Human CXCL7/NAP‑2 and cytochalasin‑B.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Myeloperoxidase Release Induced by CXCL7/NAP‑2 and Neutralization by Human CXCL7/NAP‑2 Antibody. Recombinant Human CXCL7/NAP‑2 (Catalog # 393‑NP) induces myeloperoxidase release in the cytochalasin‑B treated human neutrophils in a dose-dependent manner (orange line). Myeloperoxidase Release elicited by Recombinant Human CXCL7/NAP‑2 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL7/ NAP‑2 Monoclonal Antibody (Catalog # MAB393). The ND50 is typically 15‑20 µg/mL in the presence of cytochalasin‑B.
Detection of Human CXCL7/NAP‑2 by Simple WesternTM. Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for CXCL7/NAP‑2 at approximately 10 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human CXCL7/NAP‑2 Monoclonal Antibody (Catalog # MAB393). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CXCL7, also known as NAP-2, is a 11 kDa angiogenic alpha -chemokine that is produced by the proteolytic cleavage of the inactive 13 kDa platelet basic protein (PBP). PBP, CXCL7, and C-terminally truncated forms of CXCL7 are secreted by monocytes and platelets. Active CXCL7 binds CXCR2 and induces the chemoattration of monocytes, neutrophils, and endothelial progenitor cells. It also inhibits megakaryocytopoiesis in the bone marrow. Within the region included in this immunogen, human CXCL7 shares 52% aa sequence identity with mouse and rat CXCL7.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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