Human CXCL7/NAP-2 Antibody

Catalog # Availability Size / Price Qty
Detection of Human CXCL7/NAP‑2 by Simple WesternTM.
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Product Details
Citations (1)
Supplemental Products

Human CXCL7/NAP-2 Antibody Summary

Species Reactivity
Detects human CXCL7/NAP‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) MIP-2 is observed, 10% cross-reactivity with recombinant human (rh) GRO alpha, rhIL-8, rhCINC-2 alpha, and rhGRO beta is observed, and 5% cross-reactivity with recombinant rat (rr) CINC-1, rrCINC-2 beta, rhGCP-2, rmKC, rhMIG, and rhGRO beta. is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human CXCL7/NAP-2
Accession # P02775
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human CXCL7/NAP‑2 (Catalog # 393-NP)
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Simple Western Detection of Human CXCL7/NAP-2 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human CXCL7/NAP‑2 by Simple WesternTM. Simple Western lane view shows lysates of human blood platelets, loaded at 0.2 mg/mL. A specific band was detected for CXCL7/NAP-2 at approximately 7 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CXCL7/NAP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF393) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL7/NAP-2

Neutrophil Activating Peptide 2 (NAP-2), Connective Tissue Activating Protein III (CTAP‑III) and beta -thrombogulin ( beta ‑TG), are proteolytically processed carboxyl-terminal fragments of platelet basic protein (PBP) which is found in the alpha-granules of human platelets. NAP-2 is a member of the CXC chemokines. Similar to other ELR domain containing CXC chemokines such as IL-8 and the GRO proteins, NAP-2 has been shown to bind CXCR2 and to chemoattract and activate neutrophils. Although CTAP-III, beta -TG and PBP represent amino-terminal extended variants of NAP-2 and possess the same CXC chemokine domains, these proteins do not exhibit NAP-2 activity. It has been shown that the additional amino-terminal residues of CTAP-III masks the critical ELR receptor binding domain that is exposed on NAP-2 and may account for lack of NAP-2 activity.

  1. Schall, T. (1994) The Cytokine Handbook, 2nd edition, A. Thomson, ed. Academic Press, New York, p. 419.
  2. Malkowski, M.G. et al. (1997) J. Mol. Biol. 266:367.
Entrez Gene IDs
5473 (Human)
Alternate Names
Beta-TG; connective tissue-activating peptide III; CTAP III; CTAP3CTAP-III; CTAPIII; CXC chemokine ligand 7; C-X-C motif chemokine 7; CXCL7; CXCL7b-TG1; LA-PF4; LDGFTC1; Leukocyte-derived growth factor; low-affinity platelet factor IV; Macrophage-derived growth factor; MDGFTC2; NAP2; NAP-2; NAP-2-L1; neutrophil-activating peptide 2; neutrophil-activating peptide-2; PBPTHBGB; platelet basic protein; PPBP; pro-platelet basic protein (chemokine (C-X-C motif) ligand 7); SCAR10; SCYB7beta-thromboglobulin; small inducible cytokine B7; small inducible cytokine subfamily B, member 7; Small-inducible cytokine B7; TGB; TGB1B-TG1; THBGB1; thrombocidin 1; thrombocidin 2; thromboglobulin, beta-1

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Citation for Human CXCL7/NAP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Heme stimulates platelet mitochondrial oxidant production to induce targeted granule secretion
    Authors: GK Annarapu, D Nolfi-Done, M Reynolds, Y Wang, L Kohut, B Zuckerbrau, S Shiva
    Redox Biology, 2021;48(0):102205.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Dot Blot


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