Human CXCR1/IL-8RA Antibody (42705)

R&D Systems | Catalog # MAB330

Clone 42705 was used by HLDA to establish CD designation
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Guinea Pig, Rabbit

Applications

Validated:

Neutralization, Flow Cytometry, CyTOF-reported

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Assay Development, Bioassay, In vivo assay, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 42705
View all CXCR1/IL-8RA Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CXCR1/IL-8 RA
Met1-Leu350
Accession # AAA59159

Specificity

Detects human CXCR1/IL-8 RA transfectants but not the parental cell line. It does not cross-react with human CXCR2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CXCR1/IL-8RA Antibody (42705)

Detection of CXCR1/IL-8 RA antibody in Human Blood Granulocytes antibody by Flow Cytometry.

Detection of CXCR1/IL‑8 RA in Human Blood Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Chemotaxis Induced by CXCL8/IL‑8 and Neutralization by Human CXCR1/IL‑8 RA Antibody.

Chemotaxis Induced by CXCL8/IL‑8 and Neutralization by Human CXCR1/IL‑8 RA Antibody.

Recombinant Human CXCL8/IL-8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL8/IL-8 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330). The ND50 is typically 0.4-2.0 µg/mL.
Detection of CXCR1/IL-8RA by Flow Cytometry

Detection of CXCR1/IL-8RA by Flow Cytometry

IL‐8/CXCR1 contributes to stemness in vitro. (A) Effect on sphere formation of single agent or combination treatment with human recombinant IL‐8, anti‐CXCR1 antibody and/or repertaxin for Caki‐1 (Kruskal–Wallis test, n = 5). (B) Effect on sphere formation of single agent or combination treatment with human recombinant IL‐8, anti‐CXCR1 antibody and repertaxin for 769P cells (Kruskal–Wallis test, n = 5). (C) Flow cytometry analysis of the SP and the CXCR1+ cell compartment upon repertaxin treatment in the spheres derived from Caki‐1. (D) Histograms showing decreased SP and CXCR1+ cells upon repertaxin treatment. The yellow area indicates number of events with verapamil treatment. The red area shows CSC and CXCR1+ populations in the untreated sample. The blue area displays the remaining number of events after repertaxin treatment. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30883740), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CXCR1/IL-8RA by Western Blot

Detection of CXCR1/IL-8RA by Western Blot

IL-8 as a partial inducer of MMP9 in ESCC cells. (A): ELISA quantification of IL-8 in culture supernatants of ESCC cells after direct co-culture with macrophages. Directly co-cultured ESCC cells secreted more IL-8 than monocultured counterparts. (B): Western blot revealed the expression of known IL-8 receptors, CXCR1 (45 kDa, monomer; 80–90 kDa, dimer and their glycosylated forms) and CXCR2 (41 kDa) in TE-9, TE-10, and TE-11 cell lines. (C,D): Treatment with rhIL-8 (100 ng/mL, for 24 h) upregulated MMP9 mRNA expression in all three ESCC cell lines (C) but only triggered MMP9 secretion from TE-10 (D). Data are expressed as mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001. NS, not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37296952), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CXCR1/IL-8RA by Western Blot

Detection of CXCR1/IL-8RA by Western Blot

IL-8 as a partial inducer of MMP9 in ESCC cells. (A): ELISA quantification of IL-8 in culture supernatants of ESCC cells after direct co-culture with macrophages. Directly co-cultured ESCC cells secreted more IL-8 than monocultured counterparts. (B): Western blot revealed the expression of known IL-8 receptors, CXCR1 (45 kDa, monomer; 80–90 kDa, dimer and their glycosylated forms) and CXCR2 (41 kDa) in TE-9, TE-10, and TE-11 cell lines. (C,D): Treatment with rhIL-8 (100 ng/mL, for 24 h) upregulated MMP9 mRNA expression in all three ESCC cell lines (C) but only triggered MMP9 secretion from TE-10 (D). Data are expressed as mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001. NS, not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37296952), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CXCR1/IL-8RA Antibody (42705)

Application
Recommended Usage

CyTOF-reported

Cheng, Y. et al. (2016) J. Immunol. 196: 924. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human whole blood granulocytes

Neutralization

Measured by its ability to neutralize CXCL8/IL‑8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR1. The Neutralization Dose (ND50) is typically 0.4-2.0 µg/mL in the presence of 1 ng/mL Recombinant Human CXCL8/IL‑8.

Reviewed Applications

Read 4 reviews rated 4.5 using MAB330 in the following applications:

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCR1/IL-8RA

The human C-X-C chemokine IL-8 is a potent neutrophil chemotactic and activating factor. Two distinct G protein-linked cell surface receptors, known as IL-8 RA (type I or CXCR1) and IL-8 RB (type II or CXCR2), can interact with the IL-8 molecule. These two receptors share 77% amino acid homology. CXCR1 expression has been documented on neutrophils, monocytes, and a small population of T cells.

Long Name

Interleukin 8 Receptor A

Alternate Names

CD181, IL-8 RA, IL8RA

Entrez Gene IDs

3577 (Human); 227288 (Mouse); 54258 (Rat)

Gene Symbol

CXCR1

UniProt

AAA59159

Additional CXCR1/IL-8RA Products

Product Documents for Human CXCR1/IL-8RA Antibody (42705)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CXCR1/IL-8RA Antibody (42705)

For research use only

Citations for Human CXCR1/IL-8RA Antibody (42705)

Customer Reviews for Human CXCR1/IL-8RA Antibody (42705) (4)

4.5 out of 5
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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human CXCR1/IL-8RA Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Cervical cancer tissue
    Species: Human
    Verified Customer | Posted 11/15/2021
    Human CXCR1/IL-8RA Antibody (42705) MAB330
  • Human CXCR1/IL-8RA Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Pilomatricoma skin tumor
    Species: Human
    Verified Customer | Posted 08/09/2021
    Human CXCR1/IL-8RA Antibody (42705) MAB330
  • Human CXCR1/IL-8 RA Antibody
    Name: Michelle Chen
    Application: Block/Neutralize
    Sample Tested: Peripheral blood neutrophils
    Species: Human
    Verified Customer | Posted 04/26/2017
  • Human CXCR1/IL-8 RA Antibody
    Name: Ruhul Choudhury
    Application: Immunohistochemistry
    Sample Tested: First trimester decidua
    Species: Mouse
    Verified Customer | Posted 01/23/2017
    sodium citrate antigen retrieval
    Human CXCR1/IL-8RA Antibody (42705) MAB330

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