Human CXCR1/IL-8 RA Antibody

Clone 42705 was used by HLDA to establish CD designation
  (31 citations)
(2 Reviews)
    
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Product Details
Citations (31)
FAQs
Supplemental Products
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  • Species Reactivity
    Human
  • Specificity
    Detects human CXCR1/IL-8 RA transfectants but not the parental cell line. It does not cross-react with human CXCR2.
  • Source
    Monoclonal Mouse IgG2A Clone # 42705
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CXCR1/IL-8 RA
    Met1-Leu350
    Accession # AAA59159
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-reported
    Cheng, Y. et al. (2016) J. Immunol. 196: 924. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Measured by its ability to neutralize CXCL8/IL‑8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR1. The Neutralization Dose (ND50) is typically 0.4-2.0 µg/mL in the presence of 1 ng/mL Recombinant Human CXCL8/IL‑8.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CXCR1/IL‑8 RA in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human
CXCR1/IL‑8 RA Monoclonal Antibody (Catalog # MAB330, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Immunohistochemistry
CXCR1/IL‑8 RA in Human Endometrial Cancer Tissue. CXCR1/IL‑8 RA was detected in immersion fixed paraffin-embedded sections of human endometrial cancer tissue using Mouse Anti-Human CXCR1/IL‑8 RA Monoclonal Antibody (Catalog # MAB330) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
CXCR1/IL‑8 RA in Human Lymph Nodes. CXCR1/IL‑8 RA was detected in immersion fixed frozen sections of human lymph nodes using Mouse Anti-Human CXCR1/IL‑8 RA Monoclonal Antibody (Catalog # MAB330) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to lymphocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Chemotaxis Induced by CXCL8/IL‑8 and Neutralization by Human CXCR1/IL‑8 RA Antibody. Recombinant Human CXCL8/IL‑8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL8/IL‑8 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR1/IL‑8 RA Monoclonal Antibody (Catalog # MAB330). The ND50 is typically 0.4‑2.0 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCR1/IL-8 RA
The human C-X-C chemokine IL-8 is a potent neutrophil chemotactic and activating factor. Two distinct G protein-linked cell surface receptors, known as IL-8 RA (type I or CXCR1) and IL-8 RB (type II or CXCR2), can interact with the IL-8 molecule. These two receptors share 77% amino acid homology. CXCR1 expression has been documented on neutrophils, monocytes, and a small population of T cells.
  • Long Name:
    Interleukin 8 Receptor A
  • Entrez Gene IDs:
    3577 (Human); 227288 (Mouse); 54258 (Rat)
  • Alternate Names:
    CD128; CD181 antigen; CD181; CDw128aC-C; chemokine (C-X-C motif) receptor 1; CKR-1; CMKAR1; C-X-C chemokine receptor type 1; CXCR1; CXC-R1; CXCR-1; High affinity interleukin-8 receptor A; IL-8 receptor type 1; IL-8R A; IL8R1; IL8RA; IL-8RA; IL8RAC-C-CKR-1; IL8RBA; interleukin 8 receptor, alpha; interleukin-8 receptor type 1; interleukin-8 receptor type A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Altered monocyte differentiation and macrophage polarization patterns in patients with breast cancer
    Authors: CH Hung, FM Chen, YC Lin, ML Tsai, SL Wang, YC Chen, YT Chen, MF Hou
    BMC Cancer, 2018;18(1):366.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients
    Authors: ALT Loi, S Hoonhorst, C van Aalst, J Langereis, V Kamp, S Sluis-Eisi, N Ten Hacken, JW Lammers, L Koenderman
    Respir. Res., 2017;18(1):100.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Mycobacteria Manipulate G-Protein-Coupled Receptors to Increase Mucosal Rac1 Expression in the Lungs
    Authors: Nader Alaridah
    J Innate Immun, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  4. CXCL1 inhibits airway smooth muscle cell migration through the decoy receptor Duffy antigen receptor for chemokines.
    Authors: Al-Alwan L, Chang Y, Rousseau S, Martin J, Eidelman D, Hamid Q
    J Immunol, 2015;193(3):1416-26.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. The endothelin-integrin axis is involved in macrophage-induced breast cancer cell chemotactic interactions with endothelial cells.
    Authors: Chen C, Chen L, Hsu Y, Liu K, Fan C, Huang T
    J Biol Chem, 2014;289(14):10029-44.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Expansion of activated regulatory T cells by myeloid-specific chemokines via an alternative pathway in CSF of bacterial meningitis patients.
    Authors: Shi G, Han J, Liu G, Hao Y, Ma Y, Li T, Wu X, Zhang H, Liu Y, Wang B, Kong Y, Zhou J, Zeng H
    Eur J Immunol, 2014;44(2):420-30.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  7. Simian immunodeficiency virus and human immunodeficiency virus type 1 matrix proteins specify different capabilities to modulate B cell growth.
    Authors: Caccuri, Francesc, Giagulli, Cinzia, Reichelt, Joachim, Martorelli, Debora, Marsico, Stefania, Bugatti, Antonell, Barone, Ines, Rusnati, Marco, Guzman, Carlos A, Dolcetti, Riccardo, Caruso, Arnaldo
    J Virol, 2014;88(10):5706-17.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. The IL-8/CXCR1 axis is associated with cancer stem cell-like properties and correlates with clinical prognosis in human pancreatic cancer cases.
    Authors: Chen, Lianyu, Fan, Jie, Chen, Hao, Meng, Zhiqiang, Chen, Zhen, Wang, Peng, Liu, Luming
    Sci Rep, 2014;4(0):5911.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  9. Orphan receptor GPR15/BOB is up-regulated in rheumatoid arthritis.
    Authors: Cartwright A, Schmutz C, Askari A, Kuiper J, Middleton J
    Cytokine, 2014;67(2):53-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. Chinese herbal medicine suppresses invasion-promoting capacity of cancer-associated fibroblasts in pancreatic cancer.
    Authors: Chen, Lianyu, Qu, Chao, Chen, Hao, Xu, Litao, Qi, Qi, Luo, Jianmin, Wang, Kun, Meng, Zhiqiang, Chen, Zhen, Wang, Peng, Liu, Luming
    PLoS ONE, 2014;9(4):e96177.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  11. CXCL1/CXCL8 (GROalpha/IL-8) in human diabetic ketoacidosis plasma facilitates leukocyte recruitment to cerebrovascular endothelium in vitro.
    Authors: Omatsu T, Cepinskas G, Clarson C, Patterson E, Alharfi I, Summers K, Couraud P, Romero I, Weksler B, Fraser D
    Am J Physiol Endocrinol Metab, 2014;306(9):E1077-84.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  12. Glioblastoma stem cells are regulated by interleukin-8 signaling in a tumoral perivascular niche.
    Authors: Infanger, David W, Cho, YouJin, Lopez, Brina S, Mohanan, Sunish, Liu, S Chris, Gursel, Demirkan, Boockvar, John A, Fischbach, Claudia
    Cancer Res, 2013;73(23):7079-89.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  13. Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth.
    Authors: Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E
    Nat. Med., 2011;17(11):1498-503.
    Species: Human
    Sample Type: In Vivo
    Application: In Vivo Homing
  14. Enrichment of Foxp3+ CD4 regulatory T cells in migrated T cells to IL-6- and IL-8-expressing tumors through predominant induction of CXCR1 by IL-6.
    Authors: Eikawa S, Ohue Y, Kitaoka K
    J. Immunol., 2010;185(11):6734-40.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  15. Reduced expression of chemoattractant receptors by polymorphonuclear leukocytes in Hyper IgE Syndrome patients.
    Authors: Mintz R, Garty BZ, Meshel T, Marcus N, Katanov C, Cohen-Hillel E, Ben-Baruch A
    Immunol. Lett., 2010;130(1):97-106.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  16. CXCR1 and CXCR2 enhances human melanoma tumourigenesis, growth and invasion.
    Authors: Singh S, Nannuru KC, Sadanandam A, Varney ML, Singh RK
    Br. J. Cancer, 2009;100(10):1638-46.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  17. Spatiotemporal expression of chemokines and chemokine receptors in experimental anti-myeloperoxidase antibody-mediated glomerulonephritis.
    Authors: van der Veen BS, Petersen AH, Belperio JA, Satchell SC, Mathieson PW, Molema G, Heeringa P
    Clin. Exp. Immunol., 2009;158(1):143-53.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  18. Potential role of high-mobility group box 1 in cystic fibrosis airway disease.
    Authors: Rowe SM, Jackson PL, Liu G, Hardison M, Livraghi A, Solomon GM, McQuaid DB, Noerager BD, Gaggar A, Clancy JP, O'Neal W, Sorscher EJ, Abraham E, Blalock JE
    Am. J. Respir. Crit. Care Med., 2008;178(8):822-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  19. Expression of CXCL8, CXCR1, and CXCR2 in neurons and glial cells of the human and rabbit retina.
    Authors: Goczalik I, Ulbricht E, Hollborn M, Raap M, Uhlmann S, Weick M, Pannicke T, Wiedemann P, Bringmann A, Reichenbach A, Francke M
    Invest. Ophthalmol. Vis. Sci., 2008;49(10):4578-89.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Fresh
  20. A novel proteolytic cascade generates an extracellular matrix-derived chemoattractant in chronic neutrophilic inflammation.
    Authors: Gaggar A, Jackson PL, Noerager BD, O'Reilly PJ, McQuaid DB, Rowe SM, Clancy JP, Blalock JE
    J. Immunol., 2008;180(8):5662-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  21. Role of interleukin-8 and growth-regulated oncogene-alpha in the chemotactic migration of all-trans retinoic acid-treated promyelocytic leukemic cells toward alveolar epithelial cells.
    Authors: Tsai WH, Hsu HC, Lin CC, Ho CK, Kou YR
    Crit. Care Med., 2007;35(3):879-85.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  22. Cytokine and chemokine profiles in multiple myeloma; significance of stromal interaction and correlation of IL-8 production with disease progression.
    Authors: Kline M, Donovan K, Wellik L, Lust C, Jin W, Moon-Tasson L, Xiong Y, Witzig TE, Kumar S, Rajkumar SV, Lust JA
    Leuk. Res., 2007;31(5):591-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  23. Shear-induced capping of L-selectin on the neutrophil surface during centrifugation.
    Authors: Lee D, King MR
    J. Immunol. Methods, 2007;328(1):97-105.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  24. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006;120(2):220-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  25. Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation.
    Authors: Smith C, Damas JK, Otterdal K, ØIe E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Froland SS, Hansson GK, Aukrust P
    J. Am. Coll. Cardiol., 2006;48(8):1591-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  26. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005;175(9):6177-89.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Neut
  27. The activation of IL-8 receptors in cultured guinea pig Muller glial cells is modified by signals from retinal pigment epithelium.
    Authors: Malgorzata Goczalik I, Raap M, Weick M, Milenkovic I, Heidmann J, Enzmann V, Wiedemann P, Reichenbach A, Francke M
    J. Neuroimmunol., 2005;161(1):49-60.
    Species: Guinea Pig
    Sample Type: Whole Cells
    Application: ICC
  28. Chemokine receptor expression in rat adjuvant-induced arthritis.
    Authors: Haas CS, Martinez RJ, Attia N, Haines GK, Campbell PL, Koch AE
    Arthritis Rheum., 2005;52(12):3718-30.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  29. Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
    Authors: Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
    Blood, 2003;103(7):2806-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  30. Evasion from NK cell immunity by MHC class I chain-related molecules expressing colon adenocarcinoma.
    Authors: Doubrovina ES, Doubrovin MM, Vider E, Sisson RB, O&amp;amp;apos;Reilly RJ, Dupont B, Vyas YM
    J. Immunol., 2003;171(12):6891-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  31. Expression of functional CCR and CXCR chemokine receptors in podocytes.
    Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H
    J. Immunol., 2002;168(12):6244-52.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
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Average Rating: 4 (Based on 2 reviews)

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We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 reviews tested in 2 applications: Block/Neutralize, Immunohistochemistry.

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Images Ratings Applications Species Reviewed By Date Details
  Very Good
 Block/Neutralize Human Anonymous 04/26/2017
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Summary

Application Block/Neutralize
Sample Tested Peripheral blood neutrophils
Species Human
Immunohistochemistry Human CXCR1/IL-8 RA Antibody MAB330 Array MAB330
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Very Good
 IHC Mouse Anonymous 01/23/2017
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Image Details
Immunohistochemistry Human CXCR1/IL-8 RA Antibody MAB330
Immunohistochemistry: Human CXCR1/IL-8 RA Antibody [MAB330].

Summary

Application Immunohistochemistry
Sample Tested First trimester decidua
Species Mouse

Other Experimental Details

Other Experimental Details sodium citrate antigen retrieval

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