Human Cytosolic beta -Glucosidase/GBA3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Cytosolic beta ‑Glucosidase/GBA3 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 728702
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cytosolic beta ‑Glucosidase/GBA3
    Thr13-Leu469
    Accession # Q9H227
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Cytosolic beta ‑Glucosidase/GBA3 in HeLa Human Cell Line. Cytosolic beta ‑Glucosidase/GBA3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Cytosolic beta ‑Glucosidase/GBA3 Monoclonal Antibody (Catalog # MAB59691) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytosolic beta-Glucosidase/GBA3
There are three beta-glucosidases (GBA) in human genome. GBA1 endodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glucosylceramide (1). GBA2 encodes a microsomal beta-glucosidase that catalyzes the hydrolysis of bile acid 3-O-glucosides (2). GBA3 is a cytosolic beta-glucosidase and is predominantly expressed in liver. GBA3 efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiological beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides (3). GBA3 also has significant neutral glycosylceramidase activity, suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (4). At the protein level, GBA3 shows significant homology (>40%) with Klotho protein that is known for its association with aging process (3, 4).
  • References:
    1. Tybulewicz, V.L. et al. (1992) Nature 357:407.
    2. Matern, H. et al. (2001) J. Biol. Chem. 276:37929.
    3. de Graaf, M. et al. (2001) Biochem. J. 356:907.
    4. Hayashi, Y. et al. (2007) J. Biol. Chem. 282:30889.
  • Entrez Gene IDs:
    57733 (Human); 289687 (Rat)
  • Alternate Names:
    CBG; CBGL1; CBGL1GLUC; Cytosolic betaGlucosidase; Cytosolic beta-Glucosidase; Cytosolic beta-glucosidase-like protein 1; EC 3.2.1.21; GBA3; GLUC; glucosidase, beta, acid 3 (cytosolic); klotho-related protein; KLrP; MGC104276; MGC126878
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