Human Cytosolic beta-Glucosidase/GBA3 Antibody Summary
Accession # Q9H227
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cytosolic beta ‑Glucosidase/GBA3 in HeLa Human Cell Line. Cytosolic beta -Glucosidase/GBA3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Cytosolic beta -Glucosidase/GBA3 Monoclonal Antibody (Catalog # MAB59691) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytosolic beta-Glucosidase/GBA3
There are three beta-glucosidases (GBA) in human genome. GBA1 endodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glucosylceramide (1). GBA2 encodes a microsomal beta-glucosidase that catalyzes the hydrolysis of bile acid 3-O-glucosides (2). GBA3 is a cytosolic beta-glucosidase and is predominantly expressed in liver. GBA3 efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiological beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides (3). GBA3 also has significant neutral glycosylceramidase activity, suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (4). At the protein level, GBA3 shows significant homology (>40%) with Klotho protein that is known for its association with aging process (3, 4).
- Tybulewicz, V.L. et al. (1992) Nature 357:407.
- Matern, H. et al. (2001) J. Biol. Chem. 276:37929.
- de Graaf, M. et al. (2001) Biochem. J. 356:907.
- Hayashi, Y. et al. (2007) J. Biol. Chem. 282:30889.
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