Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC)
Measured by its ability to neutralize DcR3/TNFRSF6B-mediated inhibition of apoptosis in the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 50 ng/mL Recombinant Human DcR3/TNFRSF6B Fc Chimera, 2 ng/mL Recombinant Human Fas Ligand/TNFSF6.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
DcR3/TNFRSF6B in Human Stomach Cancer Tissue.
DcR3/TNFRSF6B was detected in immersion fixed paraffin-embedded sections of human stomach cancer using 10 µg/mL Goat Anti-Human DcR3/TNFRSF6B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF142) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the membrane of epithelial cells in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
DcR3/TNFRSF6B Inhibition of Fas Ligand/TNFSF6-induced Apoptosis and Neutralization by Human DcR3/TNFRSF6B Antibody.
In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (10 µg/mL, Catalog # MAB050), Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC) inhibits Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL) induced apoptosis in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Under these conditions, inhibition of Recombinant Human Fas Ligand/TNFSF6 (2 ng/mL) activity elicited by Recombinant Human DcR3/TNFRSF6B Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human DcR3/TNFRSF6B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF142). The ND50 is typically 0.1-0.5 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human decoy receptor 3 (DcR3), also called TNFRSF6B, TR6, and M68, is a member of the TNF receptor superfamily. The cDNA of DcR3 encodes a 300 amino acid (aa) polypeptide with a putative 23 aa signal peptide. Like osteoprotegerin (OPG), DcR3 lacks a transmembrane sequence and is a secreted protein. DcR3 shares sequence identity with OPG (31%), TNF RII (29%), and Fas (17%). It was found to be expressed in a variety of different tissues and at high levels in many malignant tumors. Among TNF family members, DcR3 was shown to bind with Fas ligand (FasL) and LIGHT and inhibit FasL- and LIGHT-induced apoptosis. Thus, in addition to DcR1, DcR2, and OPG, DcR3 is another TNFR family molecule which modulates ligands that induce apoptosis. Over-expression of DcR3 might be a mechanism by which certain tumors escape immune-cytotoxic attack.
Pitti, R.M. et al. (1998) Nature 396:699.
Yu, K-Y. et al. (1999) J. Biochem. Chem. 274:13733.
Bai, C. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1230.
Decoy Receptor 3
Entrez Gene IDs:
8771 (Human); 107126607 (Cynomolgus Monkey)
DcR3; DCR3Decoy receptor for Fas ligand; Decoy receptor 3; M68; M68DJ583P15.1.1; TNFRSF6B; TR6; TR6DcR3; tumor necrosis factor receptor superfamily member 6B; tumor necrosis factor receptor superfamily, member 6b, decoy
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.