Human DDR2 Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human DDR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human DDR1 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 290814
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human DDR2
    Gln24-Arg399
    Accession # Q16832
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human DDR2 (Catalog # 2538-DR)
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
DDR2 in Human Lung. DDR2 was detected in immersion fixed paraffin-embedded sections of human lung using 25 µg/mL Mouse Anti-Human DDR2 Monoclonal Antibody (Catalog # MAB2538) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to to cells in connective tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DDR2

DDR2, also known as TYR010 and TKT, is a widely expressed 130 kDa type I transmembrane glycoprotein belonging to the discoidin-like domain containing subfamily of receptor tyrosine kinases (1). Mature human DDR2 consists of a 378 amino acid (aa) extracellular domain (ECD) that includes the discoidin-like domain, a 22 aa transmembrane segment, and a 434 aa cytoplasmic domain that includes the kinase domain (2). Within the ECD, human DDR2 shares 53% aa sequence identity with DDR1 and 97% aa sequence identity with mouse DDR2. The discoidin-like domain mediates DDR2 interactions with collagens I, III, and X (3‑5). Collagens II and V are less efficacious ligands (3). DDR2 selectively recognizes the triple helical structure of collagen compared to monomeric or denatured collagen (3, 5, 6). Within collagen II, the D2 period is required for DDR2 binding, and the D1 period is additionally required to trigger DDR2 autophosphorylation (6). The ECD of DDR2 exists as a non-covalent dimer in solution, and dimerization of the receptor greatly enhances collagen binding (4, 7). DDR2 interaction with collagen I inhibits collagen fibrillogenesis and alters collagen fiber morphology (7). Ligand binding induces DDR2 autophosphorylation in the cytoplasmic domain (3, 5, 8), which promotes associations with Shc and Src (9). In addition to the above mechanism, DDR2 exhibits a distinct interaction with collagen X. A region other than the discoidin-like domain of DDR2 recognizes the non-helical NC1 domain of collagen X, and this interaction does not lead to receptor autophosphorylation (5). Activation of DDR2 by collagen induces upregulation of MMP-1, -2, and -13 as well as DDR2 itself (3, 8, 10). DDR2 is implicated in collagenous matrix destruction and cell invasiveness (8, 10). DDR2 is also upregulated in several pathological conditions, including hepatic fibrosis following injury, rheumatoid and osteoarthritis, and smooth muscle cell hyperplasia (8, 10‑12).

  • References:
    1. Vogel, W.F. et al. (2006) Cell. Signal. 18:1108.
    2. Karn, T. et al. (1993) Oncogene 8:3433.
    3. Vogel, W. et al. (1997) Mol. Cell 1:13.
    4. Leitinger, B. (2003) J. Biol. Chem. 278:16761.
    5. Leitinger, B. and A.P.L Kwan (2006) Matrix Biol. 25:355.
    6. Leitinger, B. et al. (2004) J. Mol. Biol. 344:993.
    7. Mihai, C. et al. (2006) J. Mol. Biol. 361:864.
    8. Olaso, E. et al. (2001) J. Clin. Invest. 108:1369.
    9. Ikeda, K. et al. (2002) J. Biol. Chem. 277:19206.
    10. Xu, L. et al. (2005) J. Biol. Chem. 280:548.
    11. Wang, J. et al. (2002) J. Autoimmun. 19:161.
    12. Ferri, N. et al. (2004) Am. J. Pathol. 164:1575.
  • Long Name:
    Discoidin Domain Receptor 2
  • Entrez Gene IDs:
    4921 (Human); 18214 (Mouse)
  • Alternate Names:
    CD167 antigen-like family member B; CD167b antigen; DDR2; Discoidin domain receptor 2; discoidin domain receptor family, member 2; discoidin domain receptor tyrosine kinase 2; discoidin domain-containing receptor 2; EC 2.7.10; EC 2.7.10.1; hydroxyaryl-protein kinase; migration-inducing gene 16 protein; neurotrophic tyrosine kinase receptor related 3; Neurotrophic tyrosine kinase, receptor-related 3; NTRKR3cell migration-inducing protein 20; Receptor protein-tyrosine kinase TKT; TKT; TKTMIG20a; Trk3; TYRO10; Tyro-10; Tyrosine-protein kinase TYRO10; tyrosylprotein kinase
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Regulation of collagen fibrillogenesis by cell-surface expression of kinase dead DDR2.
    Authors: Blissett AR, Garbellini D, Calomeni EP, Mihai C, Elton TS, Agarwal G
    J. Mol. Biol., 2009;385(3):902-11.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. Increased expression of discoidin domain receptor 2 is linked to the degree of cartilage damage in human knee joints: a potential role in osteoarthritis pathogenesis.
    Authors: Sunk IG, Bobacz K, Hofstaetter JG, Amoyo L, Soleiman A, Smolen J, Xu L, Li Y
    Arthritis Rheum., 2007;56(11):3685-92.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
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