Human Desmoglein-3 Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Desmoglein-3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Desmoglein-1, rhDesmoglein-2, or rhCadherin-17 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 216519
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Desmoglein-3
    Glu50-Arg615
    Accession # P32926
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human Desmoglein‑3 by Western Blot. Western blot shows lysates of human skin cancer (squamous cell carcinoma) tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Desmoglein‑3 Monoclonal Antibody (Catalog # MAB1720) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Desmoglein‑3 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry
Desmoglein‑3 in Human Squamous Cell Carcinoma. Desmoglein‑3 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Desmoglein‑3 Monoclonal Antibody (Catalog # MAB1720) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Desmoglein-3
Desmoglein-3, also known as pemphigus vulgaris antigen (PVA), is a 130 kDa transmembrane glycoprotein that belongs to the cadherin family of calcium dependent adhesion molecules (1‑3). Human Desmoglein-3 is synthesized with a 26 amino acid (aa) propeptide. The mature protein consists of a 566 aa extracellular domain (ECD) that contains four cadherin-like domains, a 25 aa transmembrane segment, and a 359 aa cytoplasmic domain (4). Within the ECD, human Desmoglein-3 shares 78% aa sequence identity with mouse and rat Desmoglein-3. It shares 44%, 47%, and 56% aa sequence identity with Desmoglein-1, -2, and -4 respectively. Desmoglein-3 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues (4‑6). During apoptosis, Desmoglein-3 is cleaved by caspases, plus MMP-2, and MMP-9 at sites within the cytoplasmic and extracellular regions, resulting in shortened transmembrane forms and a soluble 75 kDa ECD fragment (7, 8). The downregulation of Desmoglein-3 in oral squamous cell carcinoma correlates with metastatic potential (9). Desmoglein-3 is the target of autoantibodies in pemphigus vulgaris, a blistering skin disorder with compromised inter-keratinocyte adhesion (6, 10). Binding of these antibodies triggers Desmoglein-3 internalization and degradation by keratinocytes (10).
  • References:
    1. Kottke, M.D. et al. (2006) J. Cell Sci. 119:797.
    2. Garrod, D.R. et al. (2002) Mol. Membr. Biol. 19:81.
    3. Leckband, D. and A. Prakasam (2006) Annu. Rev. Biomed. Eng. 8:259.
    4. Amagai, M. et al. (1991) Cell 67:869.
    5. Silos, S.A. et al. (1996) J. Biol. Chem. 271:17504.
    6. Amagai, M. et al (1996) J. Invest. Dermatol. 106:351.
    7. Weiske, J. et al. (2001) J. Biol. Chem. 276:41175.
    8. Cirillo, N. et al. (2007) Oral Dis. 13:341.
    9. Wang, L. et al. (2007) Virchows Arch. Sept. 11 epub.
    10. Calkins, C.C. et al. (2006) J. Biol. Chem. 281:7623.
  • Entrez Gene IDs:
    1830 (Human)
  • Alternate Names:
    Cadherin family member 6; CDHF6; CDHF6130-kD pemphigus vulgaris antigen; desmoglein 3 (pemphigus vulgaris antigen); desmoglein 3; Desmoglein3; Desmoglein-3; DKFZp686P23184,130 kDa pemphigus vulgaris antigen; DSG3; pemphigus vulgaris antigen; PVA
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Gene expression profiling of idiopathic interstitial pneumonias (IIPs): identification of potential diagnostic markers and therapeutic targets
    Authors: Y Horimasu, N Ishikawa, M Taniwaki, K Yamaguchi, K Hamai, H Iwamoto, S Ohshimo, H Hamada, N Hattori, M Okada, K Arihiro, Y Ohtsuki, N Kohno
    BMC Med. Genet., 2017;18(1):88.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  2. Central role of alpha9 acetylcholine receptor in coordinating keratinocyte adhesion and motility at the initiation of epithelialization.
    Authors: Chernyavsky AI, Arredondo J, Vetter DE, Grando SA
    Exp. Cell Res., 2007;313(16):3542-55.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Desmoglein versus non-desmoglein signaling in pemphigus acantholysis: characterization of novel signaling pathways downstream of pemphigus vulgaris antigens.
    Authors: Chernyavsky AI, Arredondo J, Kitajima Y, Sato-Nagai M, Grando SA
    J. Biol. Chem., 2007;282(18):13804-12.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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