Detection of Human DMPK by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DMPK Monoclonal Antibody (Catalog # MAB6967) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DMPK at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
DMPK (dystrophia myotonica protein kinase), also called DMK, MDPK or MT-PK (myotonin protein kinase) is an 80 kDa cytoplasmic protein of the ser/thr kinase superfamily (EC 184.108.40.206). The 639 amino acid (aa) human DMPK includes a protein kinase domain (aa 81-349), an AGC kinase C-terminal domain (aa 350-425) and a coiled-coil negative regulatory domain that mediates dimerization (aa 467-546). Within the region used as an immunogen, human DMPK shares 72% and 73% aa sequence identity with mouse and rat DMPK, respectively. This region is included within 12 isoforms that range in size from 530-634 aa. The most prominent, muscle-specific isoform phosphorylates phospholamban and is critical for cardiac contractility.
Dystrophia Myotonica Protein Kinase
Entrez Gene IDs:
1760 (Human); 13400 (Mouse); 308405 (Rat)
DM1; DM1PK; DMK; DMKEC 220.127.116.11; DM-kinase; DMmyotonin protein kinase A; DMPK; dystrophia myotonica-protein kinase; MDPK; MDPKDM protein kinase; MT-PK; MT-PKmyotonic dystrophy associated protein kinase; Myotonic dystrophy protein kinase; myotonin-protein kinase; thymopoietin homolog
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