Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 713931
View all DMPK Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DMPK
Glu393-Thr543
Accession # Q09013

Specificity

Detects human DMPK in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human DMPK Antibody

Detection of Human DMPK antibody by Western Blot.

Detection of Human DMPK by Western Blot.

Western blot shows lysates of human heart tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DMPK Monoclonal Antibody (Catalog # MAB6967) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DMPK at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human DMPK Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human heart tissue

Reviewed Applications

Read 2 reviews rated 4.5 using MAB6967 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DMPK

DMPK (dystrophia myotonica protein kinase), also called DMK, MDPK or MT-PK (myotonin protein kinase) is an 80 kDa cytoplasmic protein of the ser/thr kinase superfamily (EC 2.7.11.1). The 639 amino acid (aa) human DMPK includes a protein kinase domain (aa 81-349), an AGC kinase C-terminal domain (aa 350-425) and a coiled-coil negative regulatory domain that mediates dimerization (aa 467-546). Within the region used as an immunogen, human DMPK shares 72% and 73% aa sequence identity with mouse and rat DMPK, respectively. This region is included within 12 isoforms that range in size from 530-634 aa. The most prominent, muscle-specific isoform phosphorylates phospholamban and is critical for cardiac contractility.

Long Name

Dystrophia Myotonica Protein Kinase

Alternate Names

DM1PK, DMK, MDPK, MT-PK

Entrez Gene IDs

1760 (Human); 13400 (Mouse); 308405 (Rat)

Gene Symbol

DMPK

UniProt

Q09013

Additional DMPK Products

Product Documents for Human DMPK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DMPK Antibody

For research use only

Related Research Areas

Intracellular Kinases

Customer Reviews for Human DMPK Antibody (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Human DMPK Antibody
    Name: NEGAR FIROOZI
    Application: Western Blot
    Sample Tested: 293T whole cell lysate and Hela whole cell lysate
    Species: Human
    Verified Customer | Posted 01/09/2023
    Western Blot Lane 1: HeLa cell lysate Lane 2: DMPK over-expressed 293 cell line Lysates/proteins at 50 µg per lane. Conditions: PVDF membrane, reducing conditions
    Human DMPK Antibody MAB6967
  • Human DMPK Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 07/27/2022
    Expected size was clear and detectable.
    Human DMPK Antibody MAB6967

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