Human DNAM-1/CD226
Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human DNAM-1/CD226 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 102511
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human DNAM-1/CD226
    Glu19-Asn247 (predicted)
    Accession # Q15762
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human DNAM-1/CD226 Fc Chimera (Catalog # 666-DN) under non-reducing conditions only
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Measured by its ability to neutralize DNAM-1/CD226-mediated adhesion of the COLO 205 human colorectal adenocarcinoma cell line. Cosman, D. et al. (1997) Immunity 7:273. The Neutralization Dose (ND50) is typically 0.1‑0.6 µg/mL in the presence of 20 µg/mL Recombinant Human DNAM-1/CD226 Fc Chimera.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of DNAM-1/CD226 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD3- cells were stained with Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB666) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Cell Adhesion Mediated by DNAM-1/CD226 and Neutral­ization by Human DNAM-1/CD226 Antibody. Recombinant Human DNAM-1/CD226 Fc Chimera (Catalog # 666-DN), immobilized onto a microplate, supports the adhesion of the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human DNAM-1/CD226 Fc Chimera (20 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB666). The ND50 is typically 0.1-0.6 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DNAM-1/CD226

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cell types and interacts with CD155/PVR and Nectin-2/CD112 (3, 4). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (2‑6), dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the formation of osteoclasts (7‑10). Platelet-endothelium interactions mediated by DNAM-1 enable the metastasis of tumor cells to the lung (11). In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation (6, 9, 12‑14).

  • References:
    1. Fuchs, A. and M. Colonna (2006) Semin. Cancer Biol. 16:359.
    2. Shibuya, A. et al. (1996) Immunity 4:573.
    3. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
    4. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
    5. Dardalhon, V. et al. (2005) J. Immunol. 175:1558.
    6. Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.
    7. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
    8. Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
    9. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
    10. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
    11. Morimoto, K. et al. (2007) Oncogene July 16 epub.
    12. Shibuya, K. et al. (1999) Immunity 11:615.
    13. Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.
    14. Shibuya, A. et al. (1998) J. Immunol. 166:1671.
  • Long Name:
    DNAX Accessory Molecule 1
  • Entrez Gene IDs:
    10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)
  • Alternate Names:
    CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1; DNAM-1; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. The Repeated Administration of Resveratrol Has Measurable Effects on Circulating T-Cell Subsets in Humans
    Authors: JL Espinoza, LQ Trung, PT Inaoka, K Yamada, DT An, S Mizuno, S Nakao, A Takami
    Oxid Med Cell Longev, 2017;2017(0):6781872.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Killing of myeloid APCs via HLA class I, CD2 and CD226 defines a novel mechanism of suppression by human Tr1 cells.
    Authors: Magnani CF, Alberigo G, Bacchetta R, Serafini G, Andreani M, Roncarolo MG, Gregori S
    Eur. J. Immunol., 2011;41(6):1652-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Mouse IgG Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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