Human DPP9 Antibody

Catalog # Availability Size / Price Qty
MAB54191-100
MAB54191-SP
Detection of Human DPP9 by Western Blot.
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Human DPP9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DPP9 in ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 988515
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human DPP9
Arg2-Leu892
Accession # Q86TI2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human DPP9 antibody by Western Blot. View Larger

Detection of Human DPP9 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human DPP9 Monoclonal Antibody (Catalog # MAB54191) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for DPP9 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry DPP9 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

DPP9 in HeLa Human Cell Line. DPP9 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human DPP9 Monoclonal Antibody (Catalog # MAB54191) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human DPP9 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human DPP9 by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue and K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for DPP9 at approximately 104 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human DPP9 Monoclonal Antibody (Catalog # MAB54191). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPP9

DPP9 is a member of the S9b family of serine peptidases (1, 2). It shares 19% amino acid identity with DPP4 and 58% amino acid identity with DPP8. It exhibits post‑proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (3). Unlike DPP4, DPP9 does not appear to be membrane bound and is localized exclusively in the cytoplasm (4). This family of proline-specific dipeptidyl peptidases has been implicated in a variety of diseases including type 2 diabetes, obesity and cancer, and has been a potential target for drug discovery (5, 6).

References
  1. Olsen, C. and Wagtmann, N. (2002) Gene 299:185.
  2. Qi, S.Y. et al. (2003) Biochem. J. 373:179.
  3. Bjelke, J.R. et al. (2006) Biochem. J. 396:391.
  4. Ajami, K. et al. (2004) Biochim. Biophys. Acta. 1679:18.
  5. Rosenblum, J.S. and Kozarich, J.W. et al. (2003) Curr. Opin. Chem. Biol. 7:496.
  6. Van der Veken, P. et al. (2007) Curr. Top. Med. Chem. 7:621.
Long Name
Dipeptidyl peptidase 9
Entrez Gene IDs
91039 (Human); 224897 (Mouse); 301130 (Rat)
Alternate Names
dipeptidyl peptidase 9; Dipeptidyl peptidase IV-related protein 2; dipeptidyl peptidase IV-related protein-2; Dipeptidyl peptidase IX; Dipeptidyl peptidase-like protein 9; dipeptidylpeptidase 9; dipeptidyl-peptidase 9; DKFZp762F117; DP9; DPLP9; DPP IX; DPP9; DPRP2; DPRP-2; EC 3.4.14.5; FLJ16073

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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