Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 988515
View all DPP9 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human DPP9
Arg2-Leu892
Accession # Q86TI2

Specificity

Detects human DPP9 in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human DPP9 Antibody

Detection of Human DPP9 antibody by Western Blot.

Detection of Human DPP9 by Western Blot.

Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human DPP9 Monoclonal Antibody (Catalog # MAB54191) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for DPP9 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

DPP9 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

DPP9 in HeLa Human Cell Line.

DPP9 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human DPP9 Monoclonal Antibody (Catalog # MAB54191) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human DPP9 antibody by Simple WesternTM.

Detection of Human DPP9 by Simple WesternTM.

Simple Western lane view shows lysates of human heart tissue and K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for DPP9 at approximately 104 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human DPP9 Monoclonal Antibody (Catalog # MAB54191). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human DPP9 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Simple Western

10 µg/mL
Sample: Human heart tissue and K562 human chronic myelogenous leukemia cell line

Western Blot

1 µg/mL
Sample:   Human heart tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DPP9

DPP9 is a member of the S9b family of serine peptidases (1, 2). It shares 19% amino acid identity with DPP4 and 58% amino acid identity with DPP8. It exhibits post‑proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (3). Unlike DPP4, DPP9 does not appear to be membrane bound and is localized exclusively in the cytoplasm (4). This family of proline-specific dipeptidyl peptidases has been implicated in a variety of diseases including type 2 diabetes, obesity and cancer, and has been a potential target for drug discovery (5, 6).

References

  1. Olsen, C. and Wagtmann, N. (2002) Gene 299:185.
  2. Qi, S.Y. et al. (2003) Biochem. J. 373:179.
  3. Bjelke, J.R. et al. (2006) Biochem. J. 396:391.
  4. Ajami, K. et al. (2004) Biochim. Biophys. Acta. 1679:18.
  5. Rosenblum, J.S. and Kozarich, J.W. et al. (2003) Curr. Opin. Chem. Biol. 7:496.
  6. Van der Veken, P. et al. (2007) Curr. Top. Med. Chem. 7:621.

Long Name

Dipeptidyl peptidase 9

Alternate Names

DPLP9, DPRP2

Entrez Gene IDs

91039 (Human); 224897 (Mouse); 301130 (Rat)

Gene Symbol

DPP9

UniProt

Q86TI2

Additional DPP9 Products

Product Documents for Human DPP9 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DPP9 Antibody

For research use only

Related Research Areas

Serine Proteases and Regulators

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Protocols

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