Human ECM-1 Antibody

Catalog # Availability Size / Price Qty
MAB39371-100
MAB39371-SP
Detection  of Human ECM‑1 by Western Blot.
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Human ECM-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ECM-1 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 966231
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ECM-1
Ala20-Glu540
Accession # Q16610
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human ECM‑1 Monoclonal Antibody (Catalog # MAB3937).

This product is intended for assay development on various assayplatforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human ECM‑1 by Western Blot. View Larger

Detection of Human ECM‑1 by Western Blot. Western blot shows lysates of CCD‑1070Sk human foreskin fibroblast cell line and WS‑1 human fetal skin fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ECM‑1 Monoclonal Antibody (Catalog # MAB39371) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ECM‑1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Human ECM‑1 ELISA Standard Curve. Recombinant human ECM-1 protein was serially diluted 2-fold and captured by Mouse Anti-Human ECM-1 Monoclonal Antibody (Catalog # MAB39371) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human ECM-1 Monoclonal Antibody (Catalog # MAB3937) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ECM1

Extracellular matrix protein-1 (ECM-1) is an 85 kDa, secreted glycoprotein important in connective tissue organization (1‑3). Of three identified splice variants the 540 amino acid (aa) form, ECM-1a, is the most widely expressed, with the highest expression in the placenta and heart (2). ECM-1b (415 aa) is found only in tonsil and associated with suprabasal keratinocytes (2, 4). Since ECM-1b expression is differentiation-dependent, a role in terminal keratinocyte differentiation has been suggested (4). ECM-1c (559 aa) accounts for approximately 15% of skin ECM-1 (5). Human ECM-1a contains a 19 aa signal peptide and a 521 aa secreted portion that includes an N-terminal proline-rich, cysteine-free region, two tandem repeat domains, and a C-terminal domain. There are six repeats of a CC(X7 ‑10)C motif (x = any aa) within the tandem repeat and C‑terminal domains. These motifs are involved in ligand binding to members of the albumin family, and are expected to form two (in ECM-1b) or three (in ECM-1a) “double loop” structures (2). Mature human ECM-1a shows 69%, 71%, 72%, and 76% aa identity with corresponding isoforms of mouse, rat, canine, and bovine ECM-1, respectively. ECM-1 is over-expressed in many malignant epithelial tumors and has demonstrated angiogenic activity (6, 7). A variety of ECM-1 mutations, mainly within the first tandem repeat, are considered causative of lipoid proteinosis, a condition showing thickened and irregular extracellular matrix within connective tissue (8). In the autoimmune condition lichen sclerosis, auto-antibodies mainly recognize the second tandem repeat or the C-terminus of ECM-1 (9). These domains also bind the extracellular matrix molecules fibulin-1 and perlecan (5, 10). The phenotypes of lipoid proteinosis and lichen sclerosis support a role for ECM-1 as a “biological glue” in the dermis (1).

References
  1. Chan, I. (2004) Exp. Dermatol. 29:52.
  2. Smits, P. et al. (1997) Genomics 45:487.
  3. Bhalerao, J. et al. (1995) J. Biol. Chem 270:16385.
  4. Smits, P. et al. (2000) J. Invest. Dermatol. 114:718.
  5. Mongiat, M. et al. (2003) J. Biol. Chem. 278:17491.
  6. Han, Z. et al. (2001) FASEB J. 15:988.
  7. Wang, L. et al. (2003) Cancer Lett. 200:57.
  8. Hamada, T. et al. (2003) J. Invest. Dermatol. 120:345.
  9. Oyama, N. et al. (2004) J. Clin. Invest. 113:1550.
  10. Fujimoto, N. et al. (2005) Biochem. Biophys. Res. Commun. 333:1327.
Long Name
Extracellular Matrix Protein 1
Entrez Gene IDs
1893 (Human); 13601 (Mouse); 116662 (Rat)
Alternate Names
ECM1; extracellular matrix protein 1; p85; Secretory Component Glycoprotein; Secretory Component P85

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG1 Isotype Control

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Reconstitution Buffers

Secondary Antibodies

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