Human ECM1 Antibody

Catalog # Availability Size / Price Qty
Human ECM‑1 ELISA Standard Curve.
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Product Details
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Human ECM1 Antibody Summary

Species Reactivity
Detects human ECM-1 in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 966248
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human ECM-1
Accession # Q16610
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human ECM‑1 Monoclonal Antibody (Catalog # MAB39371).

This product is intended for assay development on various assayplatforms requiring antibody pairs.


Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Human ECM‑1 ELISA Standard Curve. Recombinant human ECM-1 protein was serially diluted 2-fold and captured by Mouse Anti-Human ECM-1 Monoclonal Antibody (Catalog # MAB39371) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human ECM-1 Monoclonal Antibody (Catalog # MAB3937) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ECM1

Extracellular matrix protein-1 (ECM-1) is an 85 kDa, secreted glycoprotein important in connective tissue organization (1‑3). Of three identified splice variants the 540 amino acid (aa) form, ECM-1a, is the most widely expressed, with the highest expression in the placenta and heart (2). ECM-1b (415 aa) is found only in tonsil and associated with suprabasal keratinocytes (2, 4). Since ECM-1b expression is differentiation-dependent, a role in terminal keratinocyte differentiation has been suggested (4). ECM-1c (559 aa) accounts for approximately 15% of skin ECM-1 (5). Human ECM-1a contains a 19 aa signal peptide and a 521 aa secreted portion that includes an N-terminal proline-rich, cysteine-free region, two tandem repeat domains, and a C-terminal domain. There are six repeats of a CC(X7 ‑10)C motif (x = any aa) within the tandem repeat and C‑terminal domains. These motifs are involved in ligand binding to members of the albumin family, and are expected to form two (in ECM-1b) or three (in ECM-1a) “double loop” structures (2). Mature human ECM-1a shows 69%, 71%, 72%, and 76% aa identity with corresponding isoforms of mouse, rat, canine, and bovine ECM-1, respectively. ECM-1 is over-expressed in many malignant epithelial tumors and has demonstrated angiogenic activity (6, 7). A variety of ECM-1 mutations, mainly within the first tandem repeat, are considered causative of lipoid proteinosis, a condition showing thickened and irregular extracellular matrix within connective tissue (8). In the autoimmune condition lichen sclerosis, auto-antibodies mainly recognize the second tandem repeat or the C-terminus of ECM-1 (9). These domains also bind the extracellular matrix molecules fibulin-1 and perlecan (5, 10). The phenotypes of lipoid proteinosis and lichen sclerosis support a role for ECM-1 as a “biological glue” in the dermis (1).

  1. Chan, I. (2004) Exp. Dermatol. 29:52.
  2. Smits, P. et al. (1997) Genomics 45:487.
  3. Bhalerao, J. et al. (1995) J. Biol. Chem 270:16385.
  4. Smits, P. et al. (2000) J. Invest. Dermatol. 114:718.
  5. Mongiat, M. et al. (2003) J. Biol. Chem. 278:17491.
  6. Han, Z. et al. (2001) FASEB J. 15:988.
  7. Wang, L. et al. (2003) Cancer Lett. 200:57.
  8. Hamada, T. et al. (2003) J. Invest. Dermatol. 120:345.
  9. Oyama, N. et al. (2004) J. Clin. Invest. 113:1550.
  10. Fujimoto, N. et al. (2005) Biochem. Biophys. Res. Commun. 333:1327.
Long Name
Extracellular Matrix Protein 1
Entrez Gene IDs
1893 (Human); 13601 (Mouse); 116662 (Rat)
Alternate Names
ECM1; extracellular matrix protein 1; p85; Secretory Component Glycoprotein; Secretory Component P85

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