Detection of Human, Mouse, and Rat Enolase 2/Neuron‑specific Enolase by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, SH-SY5Y human neuroblastoma cell line, human brain tissue (cerebellum), mouse brain tissue (cerebellum), and rat brain tissue (cerebellum). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Enolase 2/Neuron‑specific Enolase Monoclonal Antibody (Catalog # MAB51691) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Enolase 2/Neuron‑specific Enolase at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Enolase 2/Neuron‑specific Enolase in SH‑SY5Y Human Cell Line.
Enolase 2/Neuron‑specific Enolase was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Mouse Anti-Human Enolase 2/Neuron‑specific Enolase Monoclonal Antibody (Catalog # MAB51691) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Enolase 2/Neuron-specific Enolase
Enolase 2 (2-phospho-D-glycerate hydrolyase; also Neuron-specific Enolase, NSE, neural enolase and gamma -enolase) is a 46 kDa member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Enolase 2 is 434 amino acids (aa) in length. The enzymatic site spans most of the length of the molecule. Enolase 2 exists as both a noncovalently-linked homodimer, or heterodimer with alpha -enolase. Full-length human Enolase 2 shares 99% aa identity with both mouse and canine Enolase 2. It shares 83% aa identity with human enolases 1 and 3.
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