Human EpCAM/TROP-1 Antibody MAB9601-100: R&D Systems

Human
EpCAM/TROP-1 Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human EpCAM/TROP-1 in ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 158206
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human EpCAM/TROP-1
    Extracellular domain
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
    • Human EpCAM/TROP-1 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human
    EpCAM/TROP‑1 Antibody (Catalog # MAB9601)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human EpCAM/TROP‑1 Biotinylated Antibody (Catalog # BAF960)
  • ELISA Standard
     
    Recombinant Human EpCAM/TROP1 Fc Chimera Protein, CF (Catalog # 960-EP)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of EpCAM/TROP‑1 in HT‑29 Human Cell Line by Flow Cytometry.
HT‑29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human EpCAM/TROP‑1 Monoclonal Antibody (Catalog # MAB9601, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein composed of a 242 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In human, EpCAM also shares 49% aa sequence homology with TROP-2/EGP-1. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (1). EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration (1, 2). EpCAM expression is also found to increase in human malignant neoplasias, with most carcinoma expressing EpCAM including those of arising from squamousal epithelia (1). EpCAM has been shown function as a homophilic Ca2+ independent adhesion molecule (3). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (4). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -Actinin (5).

  • References:
    1. Balzar, M. et al. (1999) J. Mol. Med. 77:699.
    2. Boer, C.J, et al. (1999) J. Pathol. 188:201.
    3. Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437.
    4. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
    5. Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.
  • Long Name:
    Epithelial Cell Adhesion Molecule
  • Entrez Gene IDs:
    4072 (Human); 17075 (Mouse)
  • Alternate Names:
    17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Soluble EpCAM levels in ascites correlate with positive cytology and neutralize catumaxomab activity in vitro.
    Authors: Seeber A, Martowicz A, Spizzo G, Buratti T, Obrist P, Fong D, Gastl G, Untergasser G
    BMC Cancer, 2015;15(0):372.
    Species: Human
    Sample Type: BALF
    Application: ELISA Capture
  2. Digital diffraction analysis enables low-cost molecular diagnostics on a smartphone.
    Authors: Im H, Castro C, Shao H, Liong M, Song J, Pathania D, Fexon L, Min C, Avila-Wallace M, Zurkiya O, Rho J, Magaoay B, Tambouret R, Pivovarov M, Weissleder R, Lee H
    Proc Natl Acad Sci U S A, 2015;112(18):5613-8.
    Species: Human
    Sample Type: Whole Cells
    Application: immunoassay
  3. Single-cell magnetic imaging using a quantum diamond microscope.
    Authors: Glenn D, Lee K, Park H, Weissleder R, Yacoby A, Lukin M, Lee H, Walsworth R, Connolly C
    Nat Methods, 2015;12(8):736-8.
    Species: Human
    Sample Type: Whole Cells
    Application: ELISA Development
  4. Rapid detection and profiling of cancer cells in fine-needle aspirates.
    Authors: Lee H, Yoon TJ, Figueiredo JL, Swirski FK, Weissleder R
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(30):12459-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Mouse IgG2B Antibody

Flow MAB0043  
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EpCAM/TROP-1 Antibody?

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