Human Ephrin-A4 Antibody

Catalog # Availability Size / Price Qty
AF369
AF369-SP
Ephrin‑A4  in Human Mammary Glands.
2 Images
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human Ephrin-A4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Ephrin-A4 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse Ephrin-A4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Ephrin-A4
Leu26-Gly171
Accession # P52798
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Ephrin-A4 Fc Chimera (Catalog # 369-EA)
Immunohistochemistry
3-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Ephrin‑A4  in Human Mammary Glands. View Larger

Ephrin‑A4 in Human Mammary Glands. Ephrin‑A4 was detected in immersion fixed paraffin-embedded sections of normal human mammary glands using Goat Anti-Human Ephrin‑A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF369) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane of glandular epithelium. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry Ephrin‑A4  in MCF‑7 Human Cell Line. View Larger

Ephrin‑A4 in MCF‑7 Human Cell Line. Ephrin‑A4 was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Human Ephrin‑A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF369) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-A4

Ephrin-A4, also known as LERK-4 and EFL-4, (1) is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A4 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1 (2, 3). The extracellular domains of human and mouse Ephrin-A4 share 80% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References
  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.
Entrez Gene IDs
1945 (Human); 13639 (Mouse)
Alternate Names
EFL4; EFL-4; EFNA4; EPH-related receptor tyrosine kinase ligand 4; EphrinA4; Ephrin-A4; EPLG4MGC125826; LERK-4; LERK4FLJ57652; ligand of eph-related kinase 4

Product Datasheets

Citations for Human Ephrin-A4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Identification of microRNA signature in the progression of gestational trophoblastic disease
    Authors: JR Zhao, WW Cheng, YX Wang, M Cai, WB Wu, HJ Zhang
    Cell Death Dis, 2018;9(2):94.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Signaling through ephrin-A ligand leads to activation of Src-family kinases, Akt phosphorylation, and inhibition of antigen receptor-induced apoptosis.
    Authors: Holen HL, Shadidi M, Narvhus K, Kjosnes O, Tierens A, Aasheim HC
    J. Leukoc. Biol., 2008;84(4):1183-91.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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Human Ephrin-A4 Antibody
By Anonymous on 10/17/2018
Application: WB Sample Tested: MDA-MB-231 human breast cancer cell line,BT-20 human breast cancer cell line Species: Human