Detection of Human Erythropoietin R by Western Blot.
Western blot shows lysates of UT‑7 human acute myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB3072) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Erythropoietin R at approximately 64 to 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Erythropoietin R
Erythropoietin (Epo), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The biological effects of Epo are mediated by the Erythropoietin Receptor (Epo R). The full length human Epo R cDNA encodes a type I membrane-spanning protein with 508 amino acid (aa) (a 24 aa hydrophobic signal sequence, a 226 aa extracellular domain, a 22 aa transmembrane domain and a 236 aa cytoplasmic domain). At the protein sequence level, the human Epo R is approximately 82% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding a truncated form of the Epo R as well as the soluble form of Epo R has been found. The presence of a soluble form of the Epo R has also been detected in human sera. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.
Barber, D.L. and A.D. D'Andrea (1992) Seminars in Hematology 29:293.
Youssoufian, H. et al. (1993) Blood 9:2223.
Lodish, H.F. et al. (1995) Cold Spring Harbor Symposia on Quantitative Biology LX:93.
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