Human Erythropoietin R Antibody (690748)

R&D Systems | Catalog # MAB3072

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 690748
View all Erythropoietin R Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Y426 of human Erythropoietin R
Accession # P19235

Specificity

Detects human Erythropoietin R in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Erythropoietin R Antibody (690748)

Detection of Human Erythropoietin R antibody by Western Blot.

Detection of Human Erythropoietin R by Western Blot.

Western blot shows lysates of UT-7 human acute myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB3072) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). Specific bands were detected for Erythropoietin R at approximately 64 to 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Erythropoietin R in KU812 cells by Flow Cytometry

KU812 cells were stained with Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB3072, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Erythropoietin R Antibody (690748)

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: KU812 Human Chronic Myelogenous Leukemic cell line

Western Blot

1 µg/mL
Sample: UT‑7 human acute myeloid leukemia cell line

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Erythropoietin R

Erythropoietin (Epo), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The biological effects of Epo are mediated by the Erythropoietin Receptor (Epo R). The full length human Epo R cDNA encodes a type I membrane-spanning protein with 508 amino acid (aa) (a 24 aa hydrophobic signal sequence, a 226 aa extracellular domain, a 22 aa transmembrane domain and a 236 aa cytoplasmic domain). At the protein sequence level, the human Epo R is approximately 82% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding a truncated form of the Epo R as well as the soluble form of Epo R has been found. The presence of a soluble form of the Epo R has also been detected in human sera. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.

References

  1. Barber, D.L. and A.D. D'Andrea (1992) Seminars in Hematology 29:293.
  2. Youssoufian, H. et al. (1993) Blood 9:2223.
  3. Lodish, H.F. et al. (1995) Cold Spring Harbor Symposia on Quantitative Biology LX:93.
  4. Baynes, R.D. et al. (1993) Blood 82:2088.

Long Name

Erythropoietin Receptor

Alternate Names

EpoR

Entrez Gene IDs

2057 (Human); 13857 (Mouse)

Gene Symbol

EPOR

UniProt

P19235

Additional Erythropoietin R Products

Product Documents for Human Erythropoietin R Antibody (690748)

Certificate of Analysis

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Product Specific Notices for Human Erythropoietin R Antibody (690748)

For research use only

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Associated Pathways

Jak/STAT Signaling Pathway
Jak/STAT Signaling Pathway Thumbnail