Human FANCD2 Antibody

Detection of Human FANCD2 by Simple Western^TM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for FANCD2 at approximately 152 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human FANCD2 Monoclonal Antibody (Catalog # MAB9369). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human FANCD2 Specificity Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FANCD2 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human FANCD2 Monoclonal Antibody (Catalog # MAB9369) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for FANCD2 at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of MMP‑9 in Human Tonsil.

Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for MMP9 mRNA (ACD RNAScope Probe, catalog #311338; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human MMP9 monoclonal antibody (R&D Systems catalog # MAB936) at 15ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes.