Human FANCD2 Antibody MAB9369-100: R&D Systems

Human FANCD2 Antibody

Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human FANCD2 in direct ELISAs and Western blots.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1290D
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    E. coli-derived recombinant human FANCD2
    Glu11-Glu230
    Accession # Q9BXW9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Simple Western
    1 µg/mL
    See below
  • Immunohistochemistry
    5-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    0.25 µg/106 cells
    See below
  • Knockout Validated
    FANCD2 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in FANCD2knockout HeLa cell line.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human FANCD2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human FANCD2 Monoclonal Antibody (Catalog # MAB9369) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for FANCD2 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
FANCD2 in Human Tonsil. FANCD2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human FANCD2 Monoclonal Antibody (Catalog # MAB9369) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Intracellular Staining by Flow Cytometry
Detection of FANCD2 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Rabbit Anti-Human FANCD2 Monoclonal Antibody (Catalog # MAB9369, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of Human FANCD2 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for FANCD2 at approximately 152 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human FANCD2 Monoclonal Antibody (Catalog # MAB9369). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Knockout Validated
Western Blot ShowsHuman FANCD2 Specificity Using Knockout Cell Line. Western blotshows lysates of HeLa human cervical epithelial carcinoma parental cell line andFANCD2 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.1 µg/mL of RabbitAnti-Human FANCD2 Monoclonal Antibody (Catalog # MAB9369) followed byHRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008).A specific band was detected for FANCD2 at approximately 150 kDa (asindicated) in the parental HeLa cell line, but is not detectable in theknockout HeLa cell line. This experiment was conducted under reducingconditions and using ImmunoblotBuffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FANCD2
Fanconi anemia group D2 protein, encoded by the FANCD2 gene, is a member of the Fanconi anemia complementation (FANC) group that also includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCE, FANCF, FANCG, and FANCL. The members of the Fanconi anemia complementation group share little sequence similarities.
  • Long Name:
    Fanconi Anemia, Complementation Group D2
  • Entrez Gene IDs:
    2177 (Human); 211651 (Mouse); 312641 (Rat)
  • Alternate Names:
    DKFZp762A223; FA4; FACD; FACDFLJ23826; FAD; FAD2; FA-D2; FADFAD2; FANCD; FANCD2; Fanconi anemia group D2 protein; Fanconi anemia, complementation group D2; Protein FACD2
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Isotype Controls
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Normal Rabbit IgG Control

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Normal Rabbit IgG Control

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Secondary Antibodies
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Rabbit IgG HRP-conjugated Antibody

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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

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Rabbit IgG PE-conjugated Antibody

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Rabbit IgG APC-conjugated Antibody

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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

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Rabbit IgG Fluorescein-conjugated Antibody

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Goat Anti-Rabbit IgG Biotinylated Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Rabbit IgG VisUCyte HRP Polymer Antibody

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Rabbit IgG Antibody

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Donkey Anti-Rabbit IgG (H+L) Antibody

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